Collagen Fiber Architecture of a Cultured Dermal Tissue

[+] Author and Article Information
M. S. Sacks

Department of Biomedical Engineering, University of Miami, P.O. Box 248294, Coral Gables, FL 33124-0621

C. J. Chuong

Biomedical Engineering Program, University of Texas at Arlington, Arlington, TX

W. M. Petroll

Department of Opthalmology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX

M. Kwan

Advanced Tissue Sciences, Inc., La Jolla, CA

C. Halberstadt

Segenix, Inc., La Jolla, CA

J Biomech Eng 119(1), 124-127 (Feb 01, 1997) (4 pages) doi:10.1115/1.2796054 History: Received September 12, 1994; Revised June 30, 1996; Online October 30, 2007


Advances in tissue engineering have led to the development of artificially grown dermal tissues for use in burn and ulcer treatments. An example of such an engineered tissue is Dermagraft™, which is grown using human neonatal fibroblasts on rectangular sheets of biodegradable mesh. Using small angle light scattering (SALS), we quantified the collagen fiber architecture of Dermagraft with the mesh scaffold contributions removed through the use of a structurally based optical model. Dermagraft collagen fibers were found to have a preferred direction nearly parallel to the long dimension of the kiteshaped mesh opening with small spatial variations over the mesh. This study demonstrated the utility of SALS as a rapid and inexpensive technique for the evaluation of gross collagen fiber architecture in engineered tissues.

Copyright © 1997 by The American Society of Mechanical Engineers
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