A Novel Injectable Collagen Matrix: In Vitro Characterization and In Vivo Evaluation

[+] Author and Article Information
Damien Laude, Kevin Odlum, Stewart Rudnicki, Nathaniel Bachrach

Organogenesis, Inc., 150 Dan Rd., Canton, MA 02021

J Biomech Eng 122(3), 231-235 (Feb 06, 2000) (5 pages) doi:10.1115/1.429653 History: Received October 21, 1999; Revised February 06, 2000
Copyright © 2000 by ASME
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Matton,  G., Anseeuw,  A., and De Keyser,  F., 1985, “The History of Injectable Biomaterials and the Biology of Collagen,” Aesthetic Plast. Surg., 9, pp. 133–140.
Cooperman,  L. S., MacKinnon,  V., Bechler,  G., and Pharriss,  B. B., 1985, “Injectable Collagen: A Six-Year Clinical Investigation,” Aesthetic Plast. Surg., 9, pp. 145–151.
Herschorn,  S., Steele,  D. J., and Radomski,  S. B., 1996, “Follow-up of Intraurethral Collagen for Female Stress Urinary Incontinence,” J. Urol. , 156, pp. 1305–1309.
Benshushan,  A., Brzezinski,  A., Shoshani,  O., and Rojansky,  N., 1998, “Periurethral Injection for the Treatment of Urinary Incontinence,” Obstet. Gynecol. Surv., 53, pp. 383–388.
Stenberg,  A., and Lackgren,  G., 1995, “A New Bioimplant for the Endoscopic Treatment of Vesicourethral Reflux: Experimental, and Short-Term Clinical Results,” J. Urol., 154, pp. 800–803.
Ford,  C. N., Martin,  D. W., and Warner,  T. F., 1984, “Injectable Collagen in Laryngeal Rehabilitation,” Laryngoscope, 94, pp. 513–518.
Wallace,  D. G., Rhee,  W., Reihanian,  H., Ksander,  G., Lee,  R., Braun,  W. B., Weiss,  B. A., and Pharriss,  B. B., 1989, “Injectable Cross-Linked Collagen With Improved Flow Properties,” J. Biomed. Mater. Res., 23, pp. 931–945.
Chan,  R. W., and Titze,  I. R., 1998, “Viscosities of Implantable Biomaterials in Vocal Fold Augmentation Surgery,” Laryngoscope, 108, pp. 725–731.
Wallace,  D. G., Condell,  R. A., Donovan,  J. W., Paivinen,  A., Rhee,  W. M., and Wade,  S. B., 1986, “Multiple Denaturational Transitions in Fibrillar Collagen,” Biopolymers, 25, pp. 1875–1895.
Chvapil,  M., Owen,  J. A., and Clark,  D. S., 1977, “Effect of Collagen Crosslinking on the Rate of Resorption of Implanted Collagen Tubing in Rabbits,” J. Biomed. Mater. Res., 11, pp. 297–314.
Vardaxis,  N. J., Ruijgrok,  J. M., Rietveld,  D. C., Marres,  E. M., and Boon,  M. E., 1994, “Chemical, and Physical Properties of Collagen Implants Influence Their Fate In Vivo as Evaluated by Light, and Confocal Microscopy,” J. Biomed. Mater. Res., 28, pp. 1013–1025.
Chvapil,  M., Holusa,  R., Kliment,  K., and Stoll,  M., 1969, “Some Chemical, and Biological Characteristics of a New Collagen–Polymer Compound Material,” J. Biomed. Mater. Res., 3, pp. 315–332.
Kemp. P. D., Falco, L., Regan, K., and Bell, E., 1992, “Collagen Compositions, and Methods for Preparation Thereof,” U.S. Patent No. 5,106,949.
Gelman,  R. A., Williams,  B. R., and Piez,  K. A., 1979, “Collagen Fibril Formation. Evidence for a Multistep Process,” J. Biol. Chem., 254, pp. 180–186.
Cavallaro,  J. F., Kemp,  P. D., and Kraus,  K. H., 1994, “Collagen Fabrics as Biomaterials,” Biotechnol. Bioeng., 43, pp. 781–791.
Lai,  W. M., and Mow,  V. C., 1980, “Drag-Induced Compression of Articular Cartilage During a Permeation Experiment,” Biorheology, 17, pp. 111–123.
Mansour,  J. M., and Mow,  V. C., 1976, “The Permeability of Articular Cartilage Under Compressive Strain, and at High Pressures,” J. Bone Joint Surg. Am., 58, pp. 509–516.
Athanasiou,  K. A., Rosenwasser,  M. P., Buckwalter,  J. A., Malinin,  T. I., and Mow,  V. C., 1991, “Interspecies Comparisons of In Situ Intrinsic Mechanical Properties of Distal Femoral Cartilage,” J. Orthop. Res., 9, pp. 330–340.
Iatridis,  J. C., Setton,  L. A., Foster,  R. J., Rawlins,  B. A., Weidenbaum,  M., and Mow,  V. C., 1998, “Degeneration Affects the Anisotropic and Nonlinear Behaviors of Human Annulus Fibrosus in Compression,” J. Biomech., 31, pp. 535–544.
Frey,  P., and Mangold,  S., 1995, “Physical, and Histological Behavior of a New Injectable Collagen (GAX 65) Implanted Into the Submucosal Space of the Mini-Pig Bladder,” J. Urol., 154, pp. 812–815.


Grahic Jump Location
The macrostructural strands of the ECF formulations are shown here prepared on a glass slide for image analysis for both the short thin strands (ST) and the long wide strands (LW). Note the distinct nature of the strands between formulations and the uniformity within each group of strands.
Grahic Jump Location
The permeability (a) and confined compression (b) testing devices shown in schematic form
Grahic Jump Location
The injectability of ECF is dependent its concentration and the needle gage used. The (*) on each main variable indicates that it has a significant effect (p<0.05, ANOVA). In both variables each level was found to be significantly different from the other two (p<0.05, N-K post hoc analysis). There was also a significant interaction found between the effect of needle gage and ECF concentration.
Grahic Jump Location
The permeability of ECF formulations was determined as a function of concentration of the ECF strands. Both short thin (ST) and long wide (LW) strand formulations of ECF reach a concentration at which permeability is measurable and beyond which the permeability decreases in a nonlinear fashion.
Grahic Jump Location
The ECF formulations were tested in confined compression in a three-stage experiment. The creep response for each stage was then fit empirically to a simple model. Typical experimental strain-time curves are shown for the low load (a), high load (b), and recovery (c) along with their respective curve fits.
Grahic Jump Location
This figure shows the in vivo persistence of the ECF formulations injected subcutaneously into rabbit ears for both; (a) the short-term mechanical compaction over a 72 hour period and (b) the long-term stability of the short thin (ST) and long wide (LW) strand ECF formulations for 330 days



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