Mechanisms of Stiffening and Strengthening in Media-Equivalents Fabricated Using Glycation

[+] Author and Article Information
T. S. Girton

Departments of Chemical Engineering & Materials Science and Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455-0132

T. R. Oegema

Orthopaedic Surgery, University of Minnesota, Minneapolis, MN 55455-0132

E. D. Grassl, B. C. Isenberg, R. T. Tranquillo

Departments of Chemical Engineering & Materials Science and Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455-0132

J Biomech Eng 122(3), 216-223 (Feb 06, 2000) (8 pages) doi:10.1115/1.429652 History: Received November 18, 1999; Revised February 06, 2000
Copyright © 2000 by ASME
Your Session has timed out. Please sign back in to continue.


Conte,  M. S., 1998, “The Ideal Small Arterial Substitute: a Search for the Holy Grail?” FASEB J., 12, pp. 43–45.
Weinberg,  C. B., and Bell,  E., 1986, “A Blood Vessel Model Constructed From Collagen and Cultured Vascular Cells,” Science, 231, pp. 397–400.
L’Heureux,  N., Germain,  L., Labbe,  R., and Auger,  F. A., 1993, “In Vitro Construction of a Human Blood Vessel From Cultured Vascular Cells: a Morphologic Study,” J. Vasc. Surg., 17, pp. 499–509.
Hirai,  J., and Matsuda,  T., 1995, “Self-Organized, Tabular Hybrid Vascular Tissue Composed of Vascular Cells and Collagen for Low-Pressure-Loaded Venous System,” Cell Transplant, 4, pp. 597–608.
Barocas,  V. H., Girton,  T. S., and Tranquillo,  R. T., 1998, “Engineered Alignment in Media-Equivalents: Magnetic Prealignment and Mandrel Compaction,” ASME J. Biomech. Eng., 120, pp. 660–666.
Girton, T. S., Oegema, T. R., and Tranquillo, R. T., 1999, “Exploiting Glycation to Stiffen and Strengthen Tissue-Equivalents for Tissue Engineering,” J. Biomed. Mat. Res., 46 .
L’Heureux,  N., Paquet,  S., Labbe,  R., Germain,  L., and Auger,  F. A., 1998, “A Completely Biological Tissue-Engineered Human Blood Vessel,” FASEB J., 12, pp. 47–56.
Esquivel,  C. O., and Blaisdell,  F. W., 1986, “Why Small Caliber Grafts Fail: a Review of Clinical and Experimental Experience and the Significance of the Interaction of Blood at the Interface,” J. Surg. Res., 41, pp. 1–15.
Barone,  L. M., Faris,  B., Chipman,  S. D., Toselli,  P., Oakes,  B. W., and Franzblau,  C., 1985, “Alteration of the Extracellular Matrix of Smooth Muscle Cells by Ascorbate Treatment,” Biochim. Biophys. Acta, 840, pp. 245–254.
Bergethon,  P. R., Mogayzel,  P. J., and Franzblau,  C., 1989, “Effect of the Reducing Environment on the Accumulation of Elastin and Collagen in Cultured Smooth-Muscle Cells,” Biochem. J., 258, pp. 279–284.
Faris,  B., Tan,  O. T., Toselli,  P., and Franzblau,  C., 1992, “Long-Term Neonatal Rat Aortic Smooth Muscle Cell Cultures: a Model for the Tunica Media of a Blood Vessel,” Matrix, 12, pp. 185–188.
Diglio,  C. A., Grammas,  P., Giacomelli,  F., and Wiener,  J., 1989, “Angiogenesis in Rat Aorta Ring Explant Cultures,” Lab Invest, 60, pp. 523–531.
Tranquillo,  R. T., Girton,  T. S., Bromberek,  B. A., Triebes,  T. G., Mooradian,  D. L., 1996, “Magnetically Oriented Tissue-Equivalent Tubes: Application to a Circumferentially Oriented Media-Equivalent,” Biomaterials, 17, pp. 349–357.
Howard,  E. W., Benton,  R., Ahern-Moore,  J., and Tomasek,  J. J., 1996, “Cellular Contraction of Collagen Lattices Is Inhibited by Nonenzymatic Glycation,” Exp. Cell Res., 228, pp. 132–137.
Barocas,  V. H., and Tranquillo,  R. T., 1997, “An Anisotropic Biphasic Theory of Tissue-Equivalent Mechanics: the Interplay Among Cell Traction, Fibrillar Network Deformation, Fibril Alignment, and Cell Contact Guidance,” ASME J. Biomech. Eng., 119, pp. 137–145.
Humason, G., 1979, Animal Tissue Techniques, 4th ed., W. H. Freeman, San Francisco.
Stegmann,  H., and Stadler,  K., 1967, “Determination of Hydroxyproline,” Clin. Chim. Acta, 18, pp. 267–273.
Chandrasekhar,  S., Esterman,  M. A., and Hoffman,  H. A., 1987, “Microdetermination of Proteoglycans and Glycosaminoglycans in the Presence of Guanidine Hydrochloride,” Anal. Biochem., 161, pp. 103–108.
Girton, T. S., 1999, “Tissue Engineering an Arterial Media-Equivalent,” Ph.D. thesis, University of Minnesota, Minneapolis, MN.
Michel,  M., Germain,  L., and Auger,  F. A., 1993, “Anchored Skin Equivalent Cultured in Vitro: a New Tool for Percutaneous Absorption Studies,” In Vitro Cell Dev. Biol., 29A, pp. 834–837.
Sajithlal,  G. B., Chithra,  P., and Chandrakasan,  G., 1998, “Advanced Glycation End Products Induce Crosslinking of Collagen in Vitro,” Biochim. Biophys. Acta, 1407, pp. 215–224.
Ozolanta,  I., Tetere,  G., Purinya,  B., and Kasyanov,  V., 1998, “Changes in the Mechanical Properties, Biochemical Contents, and Wall Structure of the Human Coronary Arteries With Age and Sex,” Med. Eng. Phys., 20, pp. 523–533.
McMahon,  M. P., , 1985, “Aging Effects on the Elastin Composition in the Extracellular Matrix of Cultured Rat Aortic Smooth Muscle Cells,” In Vitro Cell Dev. Biol., 21, pp. 674–680.
Ruckman,  J. L., Luvalle,  P. A., Hill,  K. E., Giro,  M. G., and Davidson,  J. M., 1994, “Phenotypic Stability and Variation in Cells of the Porcine Aorta: Collagen and Elastin Production,” Matrix Biol., 14, pp. 135–145.
Girton, T. S., and Tranquillo, R. T., 1999, “Confined Compression of a Tissue-Equivalent: Collagen Fibril and Cell Alignment in Response to Anisotropic Strain,” submitted.
Wolinsky,  H., and Glagov,  S., 1964, “Structural Basis for the Static Mechanical Properties of the Aortic Media,” Circ. Res., 14, pp. 400–413.
Niklason,  L. E. , 1999, “Functional Arteries Grown In Vitro,” Science, 284, pp. 489–493.
Theobald,  V. A., Lauer,  J. D., Kaplan,  F. A., Baker,  K. B., and Rosenberg,  M., 1993, “ ‘Neutral Allografts’—Lack of Allogenic Stimulation by Cultured Human Cells Expressing MHC Class I and Class II Antigen,” Transplantation, 55, pp. 128–133.
Pober,  J. S., Ma,  W., Biedermann,  B., and Libby,  P., 1997, “Vascular Cells Have Limited Capacities to Activate and Differentiate T Cells: Implications for Transplant Vascular Sclerosis,” Transpl. Immunol., 5, pp. 251–254.
Davidson,  J. M., Luvalle,  P. A., Zoia,  O., Quaglino,  D., and Giro,  M., 1997, “Ascorbate Differentially Regulates Elastin and Collagen Biosynthesis in Vascular Smooth Muscle Cells and Skin Fibroblasts by Pretranslational Mechanisms,” J. Biol. Chem., 272, pp. 345–352.
Ross,  R., 1971, “The Smooth Muscle Cell. II. Growth of Smooth Muscle in Culture and Formation of Elastic Fibers,” J. Cell Biol., 50, pp. 172–186.
Clark,  R. A., Nielsen,  L. D., Welch,  M. P., and McPherson,  J. M., 1995, “Collagen Matrices Attenuate the Collagen-Synthetic Response of Cultured Fibroblasts to TGF-Beta,” J. Cell. Sci., 108, pp. 1251–1261.
Tuan,  T. L., Song,  A., Chang,  S., Younai,  S., and Nimni,  M. E., 1996, “In Vitro Fibroplasia: Matrix Contraction, Cell Growth, and Collagen Production of Fibroblasts Cultured in Fibrin Gels,” Exp. Cell Res., 223, pp. 127–134.
Smith,  E. B., 1995, “Fibrinogen, Fibrin, and the Arterial Wall,” Eur. Heart J., 16, pp. 11–15.
Seliktar, D., Black, R. A., Vito, R. P., and Nerem, R. M., 2000, “Dynamic Mechanical Conditioning of Collage-Gel Blood Vessel Constructs Induces Remodeling In Vitro,” Ann. Biomed. Eng., in press.


Grahic Jump Location
Evolution of dry weight and collagen content of MEs: MEs incubated in standard medium for up to 10 weeks had a transient increase in dry weight but no increase in collagen content
Grahic Jump Location
Stress–strain comparison of glycated ME and arterial tissue: Circumferential stress–strain curves for MEs incubated for 6 weeks in standard medium supplemented with 30 mM ribose are more comparable to that measured for a freshly harvested abdominal aorta from an adult Fisher rat
Grahic Jump Location
Stress–strain curves for MEs fabricated with high versus low passage SMCs: Circumferential stress–strain curves for MEs fabricated with adult SMCs at passage 5 (“low”) or passage 30 (“high”) and incubated for 13 weeks in standard medium supplemented with 50 μg/ml ascorbic acid reveal MEs fabricated with the low passage adult SMCs were considerably stiffer and stronger



Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related Journal Articles
Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In