Passive Deformation Analysis of Human Leukocytes

[+] Author and Article Information
Cheng Dong, R. Skalak

Bioengineering Institute, Department of Civil Engineering and Engineering Mechanics, Columbia University, New York, NY 10027

Kuo-Li Paul Sung

Department of Physiology, College of Physicians & Surgeons, Columbia University New York, NY 10032

G. W. Schmid-Schönbein

AMES–Bioengineering, University of California, San Diego, La Jolla, CA 92093

Shu Chien

Department of Physiology, College of Physicians & Surgeons, Columbia University, New York, NY 10032

J Biomech Eng 110(1), 27-36 (Feb 01, 1988) (10 pages) doi:10.1115/1.3108402 History: Received November 16, 1987; Revised December 01, 1987; Online June 12, 2009


The following analysis presents an experimental and theoretical study of the passive viscoelastic behavior of human leukocytes. Individual neutrophils in EDTA were observed both during their partial aspiration into a small micropipette and after expulsion from a large micropipette where the cell had been totally aspirated and deformed into a sausage shape. To analyze the data, a passive model of leukocyte rheology has been developed consisting of a cortical shell containing a Maxwell fluid which describes the average properties of the cell cytoplasm. The cortical shell represents a crosslinked actin layer near the surface of the cell and is assumed to be under pre-stressed tension. This model can reproduce the results of experiments using micropipette for both short-time small deformation and slow recovery data after large deformation. In addition, a finite element scheme has been established for the same model which shows close agreement with the analytical solution.

Copyright © 1988 by ASME
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