A Flow System for the Study of Shear Forces Upon Cultured Endothelial Cells

[+] Author and Article Information
A. R. Koslow, R. R. Stromberg, L. I. Friedman

American Red Cross Biomedical Research and Development Laboratories, Bethesda, Md. 20814

R. J. Lutz

Division of Research Services, National Institutes of Health, Bethesda, Md. 20205

S. L. Hilbert, P. Schuster

Center for Devices and Radiological Health, Food and Drug Administration, Rockville, Md. 20857

J Biomech Eng 108(4), 338-341 (Nov 01, 1986) (4 pages) doi:10.1115/1.3138625 History: Received June 03, 1985; Revised July 23, 1986; Online June 12, 2009


A parallel plate chamber in a flow system has been designed to study the effects of fluid shear stresses on cells. The system was applied to the study of cultured endothelial cells grown on cover slips which were accommodated in recessed wells in the base plate. Dye injection studies in the chamber indicated laminar flow over the cells. Shear rates measured over the cover slips by an electrochemical technique were found to be linear with flow rate. Laser doppler anemometry showed parabolic profiles between the plates. Endothelial cells subjected to flow showed a correlation between the time required for orientation and the magnitude of the shear stress.

Copyright © 1986 by ASME
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