Measurement of the Water Permeability of Single Human Granulocytes on a Microscopic Stopped-Flow Mixing System

[+] Author and Article Information
K. R. Diller, D. A. Bradley

Biomedical Engineering Center, Department of Mechanical Engineering, The University of Texas, Austin, Tex. 78712

J Biomech Eng 106(4), 384-393 (Nov 01, 1984) (10 pages) doi:10.1115/1.3138510 History: Received February 01, 1983; Revised June 01, 1984; Online June 15, 2009


The water permeability (Lp ) of human granulocytes was measured for individual isolated cells with a novel, microscopic stopped-flow mixing system. Changes in volume were monitored as a cell was introduced suddently into an osmotically active solution. Permeability values were determined as a function of solution osmolality from the volume versus time curves for mixing into both hypotonic and hypertonic solutions within the range of 145 to 833 mOsm. The calculated reference permeability at 25°C was 1.15 μm/atm·min with an osmotic coefficient of 0.46 Osm/kg.

Copyright © 1984 by ASME
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