Fiber Optic Microfluorimetry for Acute and Chronic In-Vivo Animal Studies

[+] Author and Article Information
S. R. Goldstein, R. F. Bonner, R. L. Dedrick, C. C. Gibson, D. A. McGuire

Biomedical Engineering and Instrumentation Branch, Division of Research Services, National Institutes of Health, Bethesda, Md. 20205

F. H. Grantham, P. M. Gullino

Laboratory of Pathophysiology, National Cancer Institute, National Institutes of Health, Bethesda, Md. 20205

J Biomech Eng 102(3), 265-273 (Aug 01, 1980) (9 pages) doi:10.1115/1.3149584 History: Received October 29, 1979; Revised May 01, 1980; Online June 15, 2009


Highly localized long-term measurements of tumor transport properties are performed with pairs of adjacent, parallel single-strand optic fibers embedded in a laboratory animal. External illumination and light sensing equipment detect local concentration of a fluorescent indicator initially injected into the venous system. Under appropriate conditions, blood flow per unit volume, capillary permeability, and uptake of fluorescent drugs can be determined from the fluorescence time history. The observation volume has a characteristic dimension on the order of 0.5 mm. Chronic tests in 20 animals monitored for 1–2 weeks have demonstrated that the technique is practical, the tumor histology is acceptable, and the measured local tissue uptake is comparable to average values in the literature. The technique is also suitable for acute experiments using a miniature fiber optic needle probe.

Copyright © 1980 by ASME
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