Measurement of Burn-Induced Leakage of Macromolecules in Living Tissue

[+] Author and Article Information
D. M. Green, K. R. Diller

Bio-Medical Engineering Program, The University of Texas at Austin, Austin, Tex. 78712

J Biomech Eng 100(3), 153-158 (Aug 01, 1978) (6 pages) doi:10.1115/1.3426206 History: Received March 09, 1978; Online October 21, 2010


An experimental technique has been developed to measure changes in vascular permeability caused by thermal trauma to the microvascular bed of the hamster cheek pouch. A solution of flourescein isothiocyanate (FITC) conjugated dextran was injected directly into the circulatory system. Subsequent leakage of dye from vessels into the extravascular space was directly observed by light microscopy and recorded by sequential photography. Individual frames of the filmed record of the trauma and post-trauma process were digitized and analyzed by computer. A point-by-point densitometric evaluation of the digitized image provides a measure of extravascular accumulation of dye-tagged dextran. Preliminary results indicate that preburn treatment with large doses of heparin yields a significant reduction in burn-induced edema.

Copyright © 1978 by ASME
Your Session has timed out. Please sign back in to continue.





Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related Journal Articles
Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In