The first stress–strain measurements on embryonic cardiovascular tissue are described here, obtained from cyclic uniaxial loading of the primitive Ventricle. An excised ventricular segment from Hamburger/Hamilton stage-16 or stage-18 chicks (2-1/2 and 3 days of a 21-day incubation period) was mounted longitudinally between two small wires in oxygenated Krebs–Henseleit cardioplegia solution. One wire was attached to an ultrasensitive force transducer and the other to a Huxley micromanipulator controlled by remote motor drive. A real-time video tracking system calculated three myocardial surface strains based on the positions of three surface markers while the heart was deformed in a triangular wave pattern. Force transducer output was filtered, digitally sampled, and stored with strains and time. Results were plotted as strain (longitudinal, circumferential, shear, and principal) versus time, stress versus time, and stress versus longitudinal strain. The stress–strain curves were nonlinear, even at low strain levels. The hysteresis loops were large; mean hysteresis energy as a proportion of total cycle stored strain energy was 36 percent (stage 16) and 41 percent (stage 18). We created a finite element model of the ventricle and fit the model behavior to the experimental behavior to determine parameters for a stage-18 pseudoelastic strain-energy function of exponential form. The calculated exponential parameter is significantly lower than that found in corresponding uniaxial studies of mature myocardium, possibly indicating the lower fiber content of the immature tissue. The results of this study are the first step in characterizing material properties for comparisons with later developmental stages and with impaired and altered myocardium. The long-term goal is to aid in identifying the biomechanical factors regulating growth and morphogenesis.
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November 1997
Technical Papers
Passive Stress–Strain Measurements in the Stage-16 and Stage-18 Embryonic Chick Heart
C. E. Miller,
C. E. Miller
Department of Mechanical Engineering; Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642
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M. A. Vanni,
M. A. Vanni
Division of Pediatric Cardiology, Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642
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L. A. Taber,
L. A. Taber
Department of Mechanical Engineering; Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642
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B. B. Keller
B. B. Keller
Division of Pediatric Cardiology, Department of Pediatrics; Department of Physiology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642
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C. E. Miller
Department of Mechanical Engineering; Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642
M. A. Vanni
Division of Pediatric Cardiology, Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642
L. A. Taber
Department of Mechanical Engineering; Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642
B. B. Keller
Division of Pediatric Cardiology, Department of Pediatrics; Department of Physiology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642
J Biomech Eng. Nov 1997, 119(4): 445-451 (7 pages)
Published Online: November 1, 1997
Article history
Received:
May 15, 1995
Revised:
October 9, 1996
Online:
October 30, 2007
Citation
Miller, C. E., Vanni, M. A., Taber, L. A., and Keller, B. B. (November 1, 1997). "Passive Stress–Strain Measurements in the Stage-16 and Stage-18 Embryonic Chick Heart." ASME. J Biomech Eng. November 1997; 119(4): 445–451. https://doi.org/10.1115/1.2798292
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