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research-article

AN ALTERNATIVE METHOD TO CHARACTERIZE THE QUASI-STATIC, NON-LINEAR MATERIAL PROPERTIES OF MURINE ARTICULAR CARTILAGE

[+] Author and Article Information
Alexander Kotelsky

Department of Biomedical Engineering 207 Goergen Hall, Box 270168 University of Rochester Rochester, NY 14627
akotelsk@ur.rochester.edu

Chandler W. Woo

Department of Biomedical Engineering 207 Goergen Hall, Box 270168 University of Rochester Rochester, NY 14627
cwoo8@u.rochester.edu

Luis F. Delgadillo

Department of Biomedical Engineering 207 Goergen Hall, Box 270168 University of Rochester Rochester, NY 14627
ldelgadi@ur.rochester.edu

Michael S. Richards

Department of Surgery School of Medicine and Dentistry 601 Elmwood Ave., Rm 2.4153 University of Rochester Medical Center Rochester, NY 14627
Michael_Richards@urmc.rochester.edu

Mark R. Buckley

Department of Biomedical Engineering 207 Goergen Hall, Box 270168 University of Rochester Rochester, NY 14627
mark.buckley@rochester.edu

1Corresponding author.

ASME doi:10.1115/1.4038147 History: Received December 14, 2016; Revised September 19, 2017

Abstract

With the onset and progression of osteoarthritis (OA), articular cartilage (AC) mechanical properties are altered. These alterations can serve as an objective measure of tissue degradation. Although the mouse is a common and useful animal model for studying OA, it is extremely challenging to measure the mechanical properties of murine AC due to its small size (thickness < 50 um). In this study, we developed novel and direct approach to independently quantify two quasi-static mechanical properties of mouse AC: the load-dependent (non-linear) solid matrix Young’s modulus (E) and drained Poisson ratio (v). The technique involves confocal microscope-based multiaxial strain mapping of compressed, intact murine AC followed by inverse finite element analysis (iFEA) to determine E and v. Importantly, this approach yields estimates of E and v that are independent of the initial guesses used for iterative optimization. As a proof of concept, mechanical properties of AC on the medial femoral condyles of wild-type mice were obtained for both trypsin-treated and control specimens. After proteolytic tissue degradation induced through trypsin treatment, a dramatic decrease in E was observed (compared to controls) at each of 3 tested loading conditions. A significant decrease in v due to trypsin digestion was also detected. These data indicate that the method developed in this study may serve as a valuable tool for comparative studies evaluating factors involved in OA pathogenesis using experimentally-induced mouse OA models.

Copyright (c) 2017 by ASME
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