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Research Papers

Design and Fabrication of a Three-Dimensional Multi-Electrode Array for Neuron Electrophysiology

[+] Author and Article Information
Lei Zuo

Department of Mechanical Engineering,
Virginia Tech,
Blacksburg, VA 24061
e-mail: leizuo@vt.edu

Shifeng Yu

Department of Mechanical Engineering,
Virginia Tech,
Blacksburg, VA 24061

Clark A. Briggs

Department of Neuroscience,
Rosalind Franklin University,
North Chicago, IL 60064

Stanislaw Kantor

Research & Development,
AbbVie, Inc.,
1 North Waukegan Road,
North Chicago, IL 60064

Jeffery Y. Pan

Research & Development,
AbbVie, Inc.,
1 North Waukegan Rd,
North Chicago, IL 60064
e-mail: jeffrey.pan@abbvie.com

1Corresponding author.

Manuscript received February 28, 2017; final manuscript received September 10, 2017; published online October 3, 2017. Assoc. Editor: Carlijn V. C Bouten.

J Biomech Eng 139(12), 121011 (Oct 03, 2017) (6 pages) Paper No: BIO-17-1086; doi: 10.1115/1.4037948 History: Received February 28, 2017; Revised September 10, 2017

Neural recording and stimulation with high spatial and temporal resolution are highly desirable in the study of neurocommunication and diseases. Planar multiple microelectrode arrays (MEA) or quasi-three-dimensional (3D) MEA with fixed height have been proposed by many researchers and become commercially available. In this paper, we present the design, fabrication, and test of a novel true 3D multiple electrode array for brain slice stimulation and recording. This MEA is composed of 105 microelectrodes with 50 μm diameter and 125 μm center-to-center spacing integrated in a 1.2 × 1.2 mm2 area. This “true” 3D MEA allows us to precisely position the individual electrodes by piezoelectric-based actuators to penetrate the inactive tissue layer and to approach the active neurons so as to optimize the recording and stimulation of electrical field potential. The capability to stimulate nerve fibers and record postsynaptic field potentials was demonstrated in an experiment using mouse brain hippocampus slice.

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Figures

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Fig. 1

Illustration of remote actuation

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Fig. 2

(a) The displacement of the electrode during the penetration and (b) the relationship between the measured force (stress) and the displacement of (strain) the electrode

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Fig. 3

The model for the electrode position control system

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Fig. 4

The capillary array, the electrodes will sit inside the holes of this array, and the dots highlight the locations of moveable electrodes

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Fig. 5

(a) The assembly of the MEA including the MEA and the PZT controller, (b) the front view of the microwire-based electrode array after dicing(left electrode is in actuation), (c) the bottom view of the electrode array after dicing

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Fig. 6

Impedance test of the electrodes

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Fig. 7

(a) The neuron signal stimulation experiment setup, (b) recording of field excitatory postsynaptic potential at three immobile electrodes (17–19) in hippocampus area of mouse brain slice, (c) field excitatory postsynaptic potential recording using mobile elecotrodes (61, 62, and 63) in the hippocampus area of mouse brain slice

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