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Technical Brief

Biomechanical Phenotyping of the Murine Aorta: What is the Best Control?

[+] Author and Article Information
Chiara Bellini

Department of Biomedical Engineering, Yale University, New Haven, CT
c.bellini@northeastern.edu

Alexander / W Caulk

Department of Biomedical Engineering, Yale University, New Haven, CT
alexander.caulk@yale.edu

Guangxin Li

Department of Surgery, Yale School of Medicine, New Haven, CT
guangxin.li@yale.edu

George Tellides

Department of Surgery, Yale School of Medicine, New Haven, CT; Fellow of ASME, Vascular Biology and Therapeutics Program, Yale School of Medicine, New Haven, CT
george.tellides@yale.edu

Jay Humphrey

Department of Biomedical Engineering, Yale University, New Haven, CT; Fellow of ASME, Vascular Biology and Therapeutics Program, Yale School of Medicine, New Haven, CT
jay.humphrey@yale.edu

1Corresponding author.

ASME doi:10.1115/1.4035551 History: Received October 11, 2016; Revised December 16, 2016

Abstract

The availability of diverse mouse models is revealing increasingly greater information on arterial mechanics, including homeostatic adaptations and pathologic maladaptations to genetic, pharmacological, and surgical manipulations. Fundamental to understanding such biomechanical changes, however, is reliable information on appropriate control vessels. In this paper, we contrast 15 different geometrical and mechanical metrics of biaxial ascending aortic mechanics across 7 different types of possible control mice. We show that there is a comforting similarity across these multiple controls for most, though not all, metrics. In particular, three potential controls, namely non-induced conditional mice, exhibit higher values of distensibility, an important clinical metric of structural stiffness, and two potential controls exhibit higher values of intrinsic circumferential material stiffness. There is motivation, therefore, to understand better the potential changes that arise with non-induced Cre-lox or similar approaches for generating mutations conditionally. In cases of germline mutations generated by breeding heterozygous +/- mice, however, the resulting homozygous +/+ mice appear to exhibit properties similar to traditional (C57BL/6) controls.

Copyright (c) 2016 by ASME
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