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Research Papers

Characterization of Human Dental Pulp Tissue Under Oscillatory Shear and Compression

[+] Author and Article Information
Burak Ozcan, Ece Bayrak

Department of Biomedical Engineering,
TOBB University of Economics and Technology,
Ankara 06560, Turkey

Cevat Erisken

Department of Biomedical Engineering,
TOBB University of Economics and Technology,
Sogutozu Avenue No. 43, Sogutozu,
Ankara 06560, Turkey
e-mail: cerisken@etu.edu.tr

1Corresponding author.

Manuscript received February 15, 2016; final manuscript received April 19, 2016; published online May 2, 2016. Assoc. Editor: Michael Detamore.

J Biomech Eng 138(6), 061006 (May 02, 2016) (5 pages) Paper No: BIO-16-1061; doi: 10.1115/1.4033437 History: Received February 15, 2016; Revised April 19, 2016

Availability of material as well as biological properties of native tissues is critical for biomaterial design and synthesis for regenerative engineering. Until recently, selection of biomaterials and biomolecule carriers for dental pulp regeneration has been done randomly or based on experience mainly due to the absence of benchmark data for dental pulp tissue. This study, for the first time, characterizes the linear viscoelastic material functions and compressive properties of human dental pulp tissue harvested from wisdom teeth, under oscillatory shear and compression. The results revealed a gel-like behavior of the pulp tissue over the frequency range of 0.1–100 rps. Uniaxial compression tests generated peak normal stress and compressive modulus values of 39.1±20.4 kPa and 5.5±2.8 kPa, respectively. Taken collectively, the linear viscoelastic and uniaxial compressive properties of the human dental pulp tissue reported here should enable the better tailoring of biomaterials or biomolecule carriers to be employed in dental pulp regeneration.

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Figures

Grahic Jump Location
Fig. 1

Harvesting and characterization of human dental pulp tissue obtained from wisdom tooth (n = 3). Sampling of dental pulp (a); rheological characterization in PBS using a custom-made hydration chamber (b) under shear and compression (c). In (a), each space in scale bar is 1 mm.

Grahic Jump Location
Fig. 3

Compression stress–relaxation response of the native pulp tissue over 1000 s upon 20% compression at 0.05 mm/s (a) and compression response of the native pulp tissue at 10% strain (b), n = 3. In A, the solid line represents average data and the dispersions are standard deviations. Error bars represent standard deviations.

Grahic Jump Location
Fig. 2

Rheological characterization. Storage modulus, loss modulus, and tanδ versus strain amplitude behavior of native pulp tissue at 1 rps and 37 °C (a). Frequency dependence of the storage modulus (b), the loss modulus (c), and tanδ (d) of the native human dental pulp tissue at 1% strain and 37 °C. Error bars represent standard deviation, n = 3.

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