Technical Brief

Experimental Factors to Be Considered in Electroporation-Mediated Transdermal Diffusion Experiments

[+] Author and Article Information
Nataša Pavšelj

Faculty of Electrical Engineering,
University of Ljubljana,
Tržaška 25,
Ljubljana SI-1000, Slovenia
e-mail: natasa.pavselj@fe.uni-lj.si

Barbara Zorec

Faculty of Electrical Engineering,
University of Ljubljana,
Tržaška 25,
Ljubljana SI-1000, Slovenia
e-mail: barbara.zorec@fe.uni-lj.si

Damijan Miklavčič

Faculty of Electrical Engineering,
University of Ljubljana,
Tržaška 25,
Ljubljana SI-1000, Slovenia
e-mail: damijan.miklavcic@fe.uni-lj.si

Sid Becker

Mechanical Engineering Department,
University of Canterbury,
Private Bag 4800,
Christchurch 8140, New Zealand
e-mail: sid.becker@canterbury.ac.nz

1Corresponding author.

Manuscript received July 17, 2015; final manuscript received September 20, 2015; published online October 27, 2015. Assoc. Editor: Ram Devireddy.

J Biomech Eng 137(12), 124501 (Oct 27, 2015) (7 pages) Paper No: BIO-15-1353; doi: 10.1115/1.4031767 History: Received July 17, 2015; Revised September 20, 2015

In this paper, we discuss some of the primary experimental factors that should be considered when interpreting and implementing the published results of skin electroporation studies concerning measurements of mass transport across the stratum corneum (SC) in the Franz cell. It is explained that the pulse magnitude should always be considered in the context of pulse shape and that transport measurements should always be presented in the context of the trans-SC potential difference (instead of the voltage between the electrodes). The condition of the SC prior to the application of the long-duration pulse strongly influences the evolution of the local transport region (LTR). This is quantified in a simple analytical investigation of the conditions that affect the thermodynamic response of the skin.

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Grahic Jump Location
Fig. 2

(a) Schematic of the idealized LTR: a section of the SC surrounding the pre-existing pathway. The SC (thickness LSC) extends to the outer domain boundary, R0, and is traversed by a small cylindrical pore of radius RP. (b) The expansion of the LTR: the small thin-walled cylindrical region of thickness ΔR represents the expansion of the pore due to the temperature rise.

Grahic Jump Location
Fig. 1

A comparison between (a) typical HV ED and SW pulses and (b) typical LV ED and SW pulses. Note that the time scales differ by three orders of magnitude; also, the voltage scales are not the same for HV and LV.

Grahic Jump Location
Fig. 3

(a) Temperature rise dependence on pore density (inversely proportional to RO2) and (b) nonlinearity in the dependence of temperature rise on pore size, RP



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