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Research Papers

Unidirectional Cell Crawling Model Guided by Extracellular Cues

[+] Author and Article Information
Zhanjiang Wang

State Key Laboratory
of Mechanical Transmission,
Chongqing University,
Chongqing 400030, China
e-mail: wangzhanjiang001@gmail.com

Yuxu Geng

State Key Laboratory
of Mechanical Transmission,
Chongqing University,
Chongqing 400030, China

1Corresponding author.

Manuscript received February 26, 2014; final manuscript received November 28, 2014; published online January 29, 2015. Assoc. Editor: Mohammad Mofrad.

J Biomech Eng 137(3), 031006 (Mar 01, 2015) (8 pages) Paper No: BIO-14-1092; doi: 10.1115/1.4029301 History: Received February 26, 2014; Revised November 28, 2014; Online January 29, 2015

Cell migration is a highly regulated and complex cellular process to maintain proper homeostasis for various biological processes. Extracellular environment was identified as the main affecting factors determining the direction of cell crawling. It was observed experimentally that the cell prefers migrating to the area with denser or stiffer array of microposts. In this article, an integrated unidirectional cell crawling model was developed to investigate the spatiotemporal dynamics of unidirectional cell migration, which incorporates the dominating intracellular biochemical processes, biomechanical processes and the properties of extracellular micropost arrays. The interpost spacing and the stiffness of microposts are taken into account, respectively, to study the mechanism of unidirectional cell locomotion and the guidance of extracellular influence cues on the direction of unidirectional cell crawling. The model can explain adequately the unidirectional crawling phenomena observed in experiments such as “spatiotaxis” and “durotaxis,” which allows us to obtain further insights into cell migration.

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Figures

Grahic Jump Location
Fig. 1

Schematic illustration about mechanics of cell migration. (Top) the relationship between the length of stress fibers segment and interpost interval. (Bottom) the relationship between the local tension generated by stress fiber contraction and the deflection of micropost.

Grahic Jump Location
Fig. 2

Schematic illustration (top) and simulation results of spatiotemporal micropost deflection in each stage of cell migration on different micropost substrates: (a) post density D = 1 with post stiffness Ks = 1.5; (b) post density D = 2 with post stiffness Ks = 1.5; and (c) post density D = 1 with post stiffness Ks = 3.0

Grahic Jump Location
Fig. 3

Simulation results of the strain energy stored in the array of microposts on different micropost arrays: (a) post density D = 1 or 2 with the same post stiffness Ks = 1.5; (b) post stiffness Ks = 1.5 or 3.0 with the same post density D = 1; (c) post density D = 2 and post stiffness Ks = 1.5 compared with post density D = 1 and post stiffness Ks = 3.0

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