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Research Papers

Evaluation of Meniscal Mechanics and Proteoglycan Content in a Modified Anterior Cruciate Ligament Transection Model

[+] Author and Article Information
Kristine M. Fischenich

Department of Mechanical Engineering,
Colorado State University,
Campus Delivery 1374,
Fort Collins, CO 80523
e-mail: kfischenich@gmail.com

Garrett A. Coatney

Department of Mechanical Engineering,
Colorado State University,
Campus Delivery 1374,
Fort Collins, CO 80523
e-mail: gcoatney@rams.colostate.edu

John H. Haverkamp

Department of Mechanical Engineering,
Colorado State University,
Campus Delivery 1374,
Fort Collins, CO 80523
e-mail: jhhaverk@rams.colostate.edu

Keith D. Button

Orthopaedic Biomechanics Laboratories,
College of Osteopathic Medicine,
Michigan State University,
East Fee Hall 965 Fee Road, Room A-439,
East Lansing, MI 48824
e-mail: keithdbutton@gmail.com

Charlie DeCamp

Small Animal Clinical Sciences,
College of Veterinary,
Michigan State University,
784 Wilson Road, Room G-100,
East Lansing, MI 48824
e-mail: decampc@cvm.msu.edu

Roger C. Haut

Orthopaedic Biomechanics Laboratories,
College of Osteopathic Medicine,
Michigan State University,
East Fee Hall 965 Fee Road, Room A-439,
East Lansing, MI 48824
e-mail: haut@msu.edu

Tammy L. Haut Donahue

Department of Mechanical Engineering,
Colorado State University,
Campus Delivery 1374,
Fort Collins, CO 80523
e-mail: tammy.donahue@colostate.edu

1Corresponding author.

Manuscript received September 10, 2013; final manuscript received April 6, 2014; accepted manuscript posted April 22, 2014; published online May 12, 2014. Assoc. Editor: Kristen Billiar.

J Biomech Eng 136(7), 071001 (May 12, 2014) (8 pages) Paper No: BIO-13-1423; doi: 10.1115/1.4027468 History: Received September 10, 2013; Revised April 06, 2014; Accepted April 22, 2014

Post-traumatic osteoarthritis (PTOA) develops as a result of traumatic loading that causes tears of the soft tissues in the knee. A modified transection model, where the anterior cruciate ligament (ACL) and both menisci were transected, was used on skeletally mature Flemish Giant rabbits. Gross morphological assessments, elastic moduli, and glycosaminoglycan (GAG) coverage of the menisci were determined to quantify the amount of tissue damage 12 weeks post injury. This study is one of the first to monitor meniscal changes after inducing combined meniscal and ACL transections. A decrease in elastic moduli as well as a decrease in GAG coverage was seen.

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Figures

Grahic Jump Location
Fig. 1

Diagram of meniscal transections

Grahic Jump Location
Fig. 2

Safranin-O and Fast Green staining intensity: (a) no stain = 0, (b) slight staining = 1, (c) moderate staining = 2, and (d) strong staining = 3

Grahic Jump Location
Fig. 3

Representative histological images showing the image analysis for GAG coverage: (a) stitched and trimmed image was imported to scale, (b) analyze particles of whole menisci was used to determine overall area of meniscus, (c) colour deconvolution tool was used to remove area stained for GAG, and (d) analyze particles was used on the colour deconvoluted image to determine area of GAG coverage. Values from image (b) and (d) were used to determine % GAG coverage ((b − d)/b).

Grahic Jump Location
Fig. 4

Menisci 12 week's postsurgery (animals 1–6 left to right and top to bottom, all specimens are oriented identical to the first image)

Grahic Jump Location
Fig. 6

(a) Instantaneous elastic moduli by hemijoint (mean with standard error) and (b) equilibrium elastic moduli by hemijoint (mean with standard error); *denotes significant difference between control and mACLT

Grahic Jump Location
Fig. 7

GAG intensity grading (mean with standard error); *denotes significant difference between control and mACLT

Grahic Jump Location
Fig. 8

Percent area of GAG coverage by region and hemijoint (mean with standard error); *denotes significant difference between control and mACLT

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