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Research Papers

A Steady-State Mass Transfer Model of Removing CPAs From Cryopreserved Blood With Hollow Fiber Modules

[+] Author and Article Information
Weiping Ding, Xiaoming Zhou

Department of Mechanical Engineering, University of Washington, Seattle, WA 98195

Shelly Heimfeld

 Fred Hutchinson Cancer Research Center, Seattle, WA 98109

Jo-Anna Reems

 Puget Sound Blood Center, Seattle, WA 98104

Dayong Gao1

Department of Mechanical Engineering, University of Washington, Seattle, WA 98195dayong@u.washington.edu

1

Corresponding author.

J Biomech Eng 132(1), 011002 (Dec 01, 2009) (7 pages) doi:10.1115/1.4000110 History: Received November 02, 2008; Revised June 18, 2009; Posted September 01, 2009; Published December 01, 2009; Online December 01, 2009

Hollow fiber modules are commonly used to conveniently and efficiently remove cryoprotective agents (CPAs) from cryopreserved cell suspensions. In this paper, a steady-state model coupling mass transfers across cell and hollow fiber membranes is theoretically developed to evaluate the removal of CPAs from cryopreserved blood using hollow fiber modules. This steady-state model complements the unsteady-state model, which was presented in our previous study. The steady-state model, unlike the unsteady-state model, can be used to evaluate the effect of ultrafiltration flow rates on the clearance of CPAs. The steady-state model is validated by experimental results, and then is compared with the unsteady-state model. Using the steady-state model, the effects of ultrafiltration flow rates, NaCl concentrations in dialysate, blood flow rates and dialysate flow rates on CPA concentration variation and cell volume response are investigated in detail. According to the simulative results, the osmotic damage of red blood cells can easily be reduced by increasing ultrafiltration flow rates, increasing NaCl concentrations in dialysate, increasing blood flow rates, or decreasing dialysate flow rates.

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Copyright © 2010 by American Society of Mechanical Engineers
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Figures

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Figure 1

Schematic of removing CPAs with a hollow fiber module

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Figure 2

Schematic of control volume

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Figure 3

Comparisons of cell volumes and CPA concentrations calculated by steady-state and unsteady-state models

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Figure 4

Effects of ultrafiltration flow rates on cell volume and CPA concentration

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Figure 5

Effects of hypertonic NaCl dialysates on cell volume

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Figure 6

Maximal cell volumes under different blood and dialysate flow rates

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