During temporomandibular joint (TMJ) function, the mandibular condylar cartilage plays a prime role in the distribution and absorption of stresses generated over the condyle. Biomechanical characterization of the tissue under compression, however, is still incomplete. The present study investigates the regional variations in the elastic and equilibrium moduli of the condylar cartilage under high strains using unconfined compression and stress relaxation, with aims to facilitate future tissue engineering studies. Porcine condylar cartilages from five regions (anterior, central, lateral, medial, and posterior) were tested under unconfined compression. Elastic moduli were obtained from the linear regions of the stress-strain curves corresponding to the continuous deformation. Equilibrium moduli were obtained from the stress relaxation curves using the Kelvin model. The posterior region was the stiffest, followed by the middle (medial, central, and lateral) regions and the anterior region, respectively. Specifically, in terms of the equilibrium modulus, the posterior region was 1.4 times stiffer than the middle regions, which were in turn 1.7 times stiffer than the anterior region, although only the difference between anterior and posterior regions was statistically significant. No significant differences in stiffness were observed among the medial, central, lateral, and posterior regions. A positive correlation between the thickness and stiffness of the cartilage was observed, reflecting that their regional variations may be related phenomena caused in response to cartilage loading patterns. Condylar cartilage was less stiff under compression than in tension. In addition, condylar cartilage under compression appears to behave in a manner similar to the TMJ disc in terms of the magnitude of moduli and drastic initial drop in stress after a ramp strain.