Research Papers

Frictional Characteristics of Erythrocytes on Coated Glass Plates Subject to Inclined Centrifugal Forces

[+] Author and Article Information
Takashi Kandori

Graduate School of Engineering, Tohoku University, 6-6 Aramaki-Aoba, Aoba-ku, Sendai 980-8579, Japan

Toshiyuki Hayase1

Institute of Fluid Science, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan

Kousuke Inoue, Kenichi Funamoto, Takanori Takeno

Institute for International Advanced Interdisciplinary Research, International Advanced Research and Education Organization, Tohoku University, 6-3 Aramaki-Aoba, Aoba-ku, Sendai 980-8578, Japan

Makoto Ohta, Atsushi Shirai

Institute of Fluid Science, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan

Motohiro Takeda

Graduate School of Medicine, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan


Corresponding author.

J Biomech Eng 130(5), 051007 (Jul 14, 2008) (8 pages) doi:10.1115/1.2948420 History: Received August 23, 2007; Revised March 11, 2008; Published July 14, 2008

In recent years a diamond-like carbon (DLC) film and a 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer have attracted attention as coating materials for implantable artificial organs or devices. When these materials are coated on vascular devices, compatibility to blood is an important problem. The present paper focuses on friction characteristics of erythrocytes to these coating materials in a medium. With an inclined centrifuge microscope developed by the authors, observation was made for erythrocytes moving on flat glass plates with and without coating in a medium of plasma or saline under the effect of inclined centrifugal force. Friction characteristics of erythrocytes with respect to these coating materials were then measured and compared to each other to characterize DLC and MPC as coating materials. The friction characteristics of erythrocytes in plasma using the DLC-coated and noncoated glass plates are similar, changing approximately proportional to the 0.5th power of the cell velocity. The cells stick to these plates in saline as well, implying the influence of plasma protein. The results using the MPC-coated plate in plasma are similar to those of the other plates for large cell velocities, but deviate from the other results with decreased cell velocity. The results change nearly proportional to the 0.75th power of the cell velocity in the range of small velocities. The results for the MPC-coated plate in saline are similar to that in plasma but somewhat smaller, implying that the friction characteristics for the MPC-coated plate are essentially independent of plasma protein.

Copyright © 2008 by American Society of Mechanical Engineers
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Figure 1

Comparison between conventional and inclined centrifuge microscope: (a) conventional centrifuge microscope and (b) inclined centrifuge microscope

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Figure 2

Schematic of the inclined centrifuge microscope system

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Figure 3

Principle of friction force measurement in a rotating field: (a) initial state and (b) steady-state movement of erythrocytes

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Figure 4

Sample plates (small division=1mm): (a) MPC-coated plate, (b) DLC-coated plate, and (c) noncoated plate

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Figure 5

Roughness of sample plates measured by AFM: (a) noncoated plate and (b) DLC-coated plate

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Figure 6

Behavior of erythrocytes in plasma on MPC-coated plate (FT=35pN, FN=47pN)

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Figure 7

Behavior of erythrocytes in saline (FT=30pN, FN=47pN): (a) MPC-coated plate and (b) DLC-coated plate

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Figure 8

Behavior of erythrocytes in plasma on noncoated plate (FT=30pN, FN=47pN).

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Figure 9

Variation of mean value and standard deviation of velocity of erythrocytes in plasma on noncoated plate (FT=30pN, FN=47pN): (a) mean value and (b) standard deviation

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Figure 10

Cell velocity in plasma with tangential force. Open symbols: plasma; closed symbols: saline; error bar represents standard deviation. (FN=47pN): (a) MPC-coated plate, (b) DLC-coated plate, and (c) noncoated plate.

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Figure 11

Friction characteristics of erythrocytes: (a) friction and equivalent shear rate and (b) equivalent gap width




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