0
Research Papers

# P188 Reduces Cell Death and IGF-I Reduces GAG Release Following Single-Impact Loading of Articular Cartilage

[+] Author and Article Information
Roman M. Natoli

Department of Bioengineering, Rice University, 6100 Main Street, Keck Hall Suite 116, Houston, TX 77005; MSTP, Baylor College of Medicine, One Baylor Plaza, N201, Houston, TX 77030

Kyriacos A. Athanasiou1

Department of Bioengineering, Rice University, 6100 Main Street, Keck Hall Suite 116, Houston, TX 77005

1

Corresponding author.

J Biomech Eng 130(4), 041012 (Jun 11, 2008) (9 pages) doi:10.1115/1.2939368 History: Received June 19, 2007; Revised February 18, 2008; Published June 11, 2008

## Abstract

Prior joint injury predisposes an individual to developing post-traumatic osteoarthritis, for which there is presently no disease modifying treatment. In this condition, articular cartilage degenerates due to cell death and matrix breakdown, resulting in tissue with diminished biomechanical function. P188, a nonionic surfactant, and the growth factor IGF-I have been shown to decrease cell death. Additionally, IGF-I is known to have beneficial effects on cartilage matrix. The objective of this study was to determine the efficacy of P188, IGF-I, and their combination following articular cartilage impact injury with two energy levels, $1.1J$ (“low”) and $2.8J$ (“high”), at $24h$ and $1week$. Bovine articular cartilage with attached underlying bone was impacted at the low or high level. Impact sites were explanted and examined immediately, or cultured for $24h$ or $1week$ in serum-free media supplemented with P188 $(8mg∕ml)$, IGF-I $(100ng∕ml)$, or their combination. Gross morphology, cell viability, GAG release to the media, and tissue mechanical properties were assessed. Immediately postimpact, high level impacted tissue had significantly increased gross morphology scores, indicating tissue damage, which were maintained over $1week$. Gross scores following low impact were initially similar to nonimpacted controls, but, at $24h$ and $1week$, low impact gross scores significantly increased compared to nonimpacted controls. Additionally, at $24h$, high impact resulted in increased cell death, and both low and high impacts had increased GAG release compared to nonimpacted controls. Furthermore, high impact caused decreased tissue stiffness at $24h$ that appeared to worsen over $1week$, evident by the percent decrease from nonimpacted controls increasing from 16% to 26%. No treatment type studied mitigated this loss. The combination did not perform better than either individual treatment; however, following low impact at $1week$, P188 reduced cell death by 75% compared to no treatment and IGF-I decreased GAG release from the tissue by 49%. In conclusion, high impact resulted in immediate tissue changes that worsened over $1week$. Though not causing immediate changes, low impact also resulted in tissue degeneration evident by $24h$. No treatment studied was effective at $24h$, but by $1week$ P188 and IGF-I ameliorated established detrimental changes occurring in articular cartilage postimpact. However, further work is needed to optimize treatment strategies to prevent and/or reverse cell death and matrix destruction in a way that maintains tissue mechanical properties, and hence its functionality.

<>

## Figures

Figure 1

Response of articular cartilage immediately postinjury. (a) Gross morphology scores. * denotes high impact significantly different from nonimpacted control and low impact groups (p<0.05). (b) Cell viability. (c) Tissue stiffness (aggregate modulus). (d) Representative gross morphology and cell viability images obtained in this study. Each bar represents mean±SD for n=5–6.

Figure 2

Response of articular cartilage 24h postinjury. (a) Gross morphology scores. (b) Cell viability. (c) Tissue stiffness (aggregate modulus). (d) GAG release. Within a panel, groups not connected by the same letter are significantly different from one another (lower case reserved for impact level and upper case for treatment type); * denotes significant difference from all other levels of the same factor (p<0.05). Each bar represents mean±SD for n=10–12.

Figure 3

Response of articular cartilage 1week postinjury. (a) Gross morphology scores. (b) Cell viability. (c) Tissue stiffness (aggregate modulus). (d) GAG release. Within a panel, groups not connected by the same letter are significantly different from one another (lower case reserved for impact level and upper case for treatment type); * and † denote significant difference from all other levels of the same factor (p<0.05). Each bar represents mean±SD for n=10–12.

## Errata

Some tools below are only available to our subscribers or users with an online account.

### Related Content

Customize your page view by dragging and repositioning the boxes below.

Related Journal Articles
Related Proceedings Articles
Related eBook Content
Topic Collections