To understand brain injuries better, the mechanical properties of brain tissue have been studied for ; however, no universally accepted data set exists. The variation in material properties reported may be caused by differences in testing methods and protocols used. An overview of studies on the mechanical properties of brain tissue is given, focusing on testing methods. Moreover, the influence of important test conditions, such as temperature, anisotropy, and precompression was experimentally determined for shear deformation. The results measured at room temperature show a stiffer response than those measured at body temperature. By applying the time-temperature superposition, a horizontal shift factor was found, which is in agreement with the values found in literature. Anisotropy of samples from the corona radiata was investigated by measuring the shear resistance for different directions in the sagittal, the coronal, and the transverse plane. The results measured in the coronal and the transverse plane were 1.3 and 1.25 times stiffer than the results obtained from the sagittal plane. The variation caused by anisotropy within the same plane of individual samples was found to range from 25% to 54%. The effect of precompression on shear results was investigated and was found to stiffen the sample response. Combinations of these and other factors (postmortem time, donor age, donor type, etc.) lead to large differences among different studies, depending on the different test conditions.