0
Research Papers

Nanoindentation Measurements of Biomechanical Properties in Mature and Newly Formed Bone Tissue Surrounding an Implant

[+] Author and Article Information
Romain Vayron

Laboratoire de Modélisation et de Simulation Multi-Echelle, UMR CNRS 8208,  CNRS, Université Paris Est, 61 Avenue du Général de Gaulle, Créteil 94010, France

Etienne Barthel

Surface du Verre et Interfaces, UMR CNRS 125, CNRS, Saint-Gobain, 39, quai Lucien Lefranc, Aubervilliers 93303, France

Vincent Mathieu

Laboratoire de Biomécanique Biomatériau Ostéo Articulaire,  CNRS, Université Paris 7, UMR CNRS 7052, 10 Avenue de Verdun, Paris 75010, France

Emmanuel Soffer, Fani Anagnostou

Laboratoire de Biomécanique Biomatériau Ostéo Articulaire and Department of Periodontology, Service of Odontology, Pitié Salpetrière Hospital et Hôtel-Dieu Hospital AP-HP,U.F.R. of Odontology,  Université Paris 7, 5 rue Garancière, 75006 Paris, France

Guillaume Haiat1

Laboratoire de Modélisation et de Simulation Multi-Echelle,UMR CNRS 8208,  CNRS, Université Paris Est, 61 Avenue du Général de Gaulle, Créteil 94010, Franceguillaume.haiat@univ-paris-est.fr

1

Corresponding author.

J Biomech Eng 134(2), 021007 (Mar 19, 2012) (6 pages) doi:10.1115/1.4005981 History: Received August 24, 2011; Revised February 01, 2012; Posted February 13, 2012; Published March 14, 2012; Online March 19, 2012

The characterization of the biomechanical properties of newly formed bone tissue around implants is important to understand the osseointegration process. The objective of this study is to investigate the evolution of the hardness and indentation modulus of newly formed bone tissue as a function of healing time. To do so, a nanoindentation device is employed following a multimodality approach using histological analysis. Coin-shaped implants were placed in vivo at a distance of 200 μm from the cortical bone surface, leading to an initially empty cavity of 200 μm * 4.4 mm. Three New Zealand White rabbits were sacrificed after 4, 7, and 13 weeks of healing time. The bone samples were embedded and analyzed using histological analyses, allowing to distinguish mature and newly formed bone tissue. The bone mechanical properties were then measured in mature and newly formed bone tissue. The results are within the range of hardness and apparent Young’s modulus values reported in previous literature. One-way ANOVA test revealed a significant effect of healing time on the indentation modulus (p < 0.001, F = 111.24) and hardness (p < 0.02, F = 3.47) of bone tissue. A Tukey-Kramer analysis revealed that the biomechanical properties of newly formed bone tissue (4 weeks) were significantly different from those of mature bone tissue. The comparison with the results obtained in Mathieu (2011, “Micro-Brillouin Scattering Measurements in Mature and Newly Formed Bone Tissue Surrounding an Implant,” J. Biomech. Eng., 133 , 021006). shows that bone mass density increases by approximately 13.5% between newly formed bone (7 weeks) and mature bone tissue.

FIGURES IN THIS ARTICLE
<>
Copyright © 2012 by American Society of Mechanical Engineers
Your Session has timed out. Please sign back in to continue.

References

Figures

Grahic Jump Location
Figure 2

High speed cutter used with physiological saline solution irrigation to level a 5.6 mm diameter bone surface and to ensure a constant gap between the implant and bone

Grahic Jump Location
Figure 3

Image taken after histological preparation. The gray scale corresponds to the degree of mineralization of bone and allows to discriminate newly formed bone tissue (less mineralized, brighter) from mature bone tissue (more mineralized, darker).

Grahic Jump Location
Figure 4

Image with indents in newly formed and mature bone tissue. The gray scale shows the degree of mineralization of bone and allows to discriminate the two types of bone tissue.

Grahic Jump Location
Figure 1

Schematic representation of the coin-shaped implant model

Tables

Errata

Discussions

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related Journal Articles
Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In