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Theoretical Study on Temperature Dependence of Cellular Uptake of QDs Nanoparticles

[+] Author and Article Information
Aili Zhang, Yingxue Guan

 School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, P. R. C.

Lisa X. Xu1

 School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, P. R. C.; Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, P. R. C.e-mail: lisaxu@sjtu.edu.cn

1

Corresponding author.

J Biomech Eng 133(12), 124502 (Dec 21, 2011) (6 pages) doi:10.1115/1.4005481 History: Received June 15, 2011; Revised November 17, 2011; Published December 21, 2011; Online December 21, 2011

Cellular uptake kinetics of nanoparticles is one of the key issues determining the design and application of the particles. Models describing nanoparticles intrusion into the cell mostly take the endocytosis process into consideration, and the influences of electrical charges, sizes, concentrations of the particles have been investigated. In this paper, the temperature effect on the cellular uptake of Quantum Dots (QDs) is studied experimentally. QDs are incubated with the SPCA-1 human lung tumor cells, and the nanoparticles on the cell membrane and inside the cell are quantified according to the fluorescence intensities recorded. It is found that the amounts of nanoparticles attached onto the cell membrane and inside the cell both increase with temperature. Based on the experimental results, a model is proposed to describe the cellular uptake dynamic process of nanoparticles. The process consists of two steps: nanoparticles adsorption onto the cell membrane and the internalization. The dynamic parameters are obtained through curve fitting. The simulated results show that the internalization process can be categorized into different phases. The temperature dependent internalization rate constant is very small when below 14 °C. It increases distinctly when temperature rises from 14 °C to 22 °C, but there is no evident increase as temperature further increases above 22 °C. Results show that by incorporating a temperature-independent internalization factor, the model predictions well fit the experimental results.

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Copyright © 2011 by American Society of Mechanical Engineers
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Figures

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Figure 2

The enlarged images of the SPCA-1 cells incubated with QDs at 37 °C for different time: (a) 10 min, (b) 3 h. Scale bar: 1 μ m.

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Figure 3

Fluorescence images of QDs in SPCA-1 cells after being co-cultured at different temperatures for 3 h. (a) 6 °C, (b) 14 °C, (c) 22 °C, (d) 26.5 °C, (e) 30 °C, (f) 37 °C. Scale bar: 20 μ m.

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Figure 4

The QD fluorescence intensity of SPCA-1 cells at different temperatures 6 °C, 22 °C and 37 °C: (a) the intensities of nanoparticles attached on cell membrane; (b) the intensities of nanoparticles inside cells. Symbols are experimental results, and the solid lines are the fitted curves (n = 18, n is the number of images averaged for each curve).

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Figure 5

The two-step process of nanoparticle cellular uptake

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Figure 6

The adsorption and internalization rate constants obtained at different temperatures: (a) ka ; (b) kd . The solid lines are the fitted curves of the rate constants with respect to temperature as: ka(T)=594.7244-5.70103×eT/16.02728(R2 (ka ) = 0.99192), kd(T)=11.7312+0.010088T<>+ 0.00445T2(R2 (kd ) = 0.9954).

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Figure 7

The internalization rate constants ki at different temperatures. The solid line is the fitted curve of the rate constant.

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Figure 8

The maximum fluorescence values of temperature-independent (mint10 ) and temperature-dependent internalized (mint20 ) nanoparticles at different temperatures

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Figure 1

Fluorescence Images of SPCA-1 cells co-cultured with QDs at 37 °C for different time intervals: (a) 10 min, (b) 30 min, (c) 1 h, (d) 2 h, and (e) 3 h. Scale bar: 20 μ m.

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