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Technical Briefs

Novel Technique for Online Characterization of Cartilaginous Tissue Properties

[+] Author and Article Information
Tai-Yi Yuan

Department of Biomedical Engineering, Tissue Biomechanics Laboratory, College of Engineering,  University of Miami, Coral Gables, FL 33146

Chun-Yuh Huang

Department of Biomedical Engineering, Stem Cell and Mechanobiology Laboratory, College of Engineering,  University of Miami, Coral Gables, FL 33146

Wei Yong Gu1

Department of Biomedical Engineering, Tissue Biomechanics Laboratory; Department of Mechanical and Aerospace Engineering, College of Engineering,  University of Miami, Coral Gables, FL 33146 wgu@miami.edu

1

Corresponding author.

J Biomech Eng 133(9), 094504 (Oct 11, 2011) (4 pages) doi:10.1115/1.4004920 History: Received July 05, 2011; Accepted August 16, 2011; Published October 11, 2011; Online October 11, 2011

The goal of tissue engineering is to use substitutes to repair and restore organ function. Bioreactors are an indispensable tool for monitoring and controlling the unique environment for engineered constructs to grow. However, in order to determine the biochemical properties of engineered constructs, samples need to be destroyed. In this study, we developed a novel technique to nondestructively online-characterize the water content and fixed charge density of cartilaginous tissues. A new technique was developed to determine the tissue mechano-electrochemical properties nondestructively. Bovine knee articular cartilage and lumbar annulus fibrosus were used in this study to demonstrate that this technique could be used on different types of tissue. The results show that our newly developed method is capable of precisely predicting the water volume fraction (less than 3% disparity) and fixed charge density (less than 16.7% disparity) within cartilaginous tissues. This novel technique will help to design a new generation of bioreactors which are able to actively determine the essential properties of the engineered constructs, as well as regulate the local environment to achieve the optimal conditions for cultivating constructs.

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Copyright © 2011 by American Society of Mechanical Engineers
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Figures

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Figure 1

Schematic of the apparatus for online-characterization of tissue properties. The apparatus consists of a perfusion chamber and measurement/control systems to monitor the cartilaginous tissue properties in real-time.

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Figure 2

Schematic of streaming potential response to applied pressure. The streaming potential is determined as the peak-to-peak value, i.e., the difference between S1 and S2 points.

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Figure 3

Comparison of water volume fraction of articular cartilage (AC) and annulus fibrosus (AF) determined by this new online technique and by the lyophilizing method

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Figure 4

Comparison of FCD of articular cartilage (AC) and annulus fibrosus (AF) determined by the online streaming potential method and DMMB method

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