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Research Papers

Effect of Storage Duration on the Mechanical Behavior of Mouse Carotid Artery

[+] Author and Article Information
Mazyar Amin

Department of Biomedical Engineering,  St. Louis University, 3507 Lindell Blvd., St. Louis, MO 63103mamin2@slu.edu

Amber G. Kunkel

Department of Computational and Applied Math  Rice University, 6350 Main St., Houston, TX 77005amber.g.kunkel@rice.edu

Victoria P. Le

Department of Biomedical Engineering,  St. Louis University, 3507 Lindell Blvd., St. Louis, MO 63103vle2@slu.edu

Jessica E. Wagenseil1

Department of Biomedical Engineering,  St. Louis University, 3507 Lindell Blvd., St. Louis, MO 63103jwagense@slu.edu

1

Corresponding author.

J Biomech Eng 133(7), 071007 (Jul 18, 2011) (6 pages) doi:10.1115/1.4004415 History: Received April 14, 2011; Accepted May 27, 2011; Revised May 27, 2011; Published July 18, 2011; Online July 18, 2011

Determining arterial mechanical properties is important for understanding the work done by the heart and how it changes with cardiovascular disease. Ex vivo tests are necessary to apply various loads to the artery and obtain data to model and predict the behavior under any load. Most ex vivo tests are performed within 24 h of dissection, so the tissue is still “alive.” For large elastic arteries; however, the passive mechanical behavior is attributed mostly to the very stable proteins, elastin, and collagen. If the testing equipment fails, is in use, or is located at another facility, it would be useful to store the vessels and postpone the tests until the equipment is available. The goal of this study is to determine the effects of storage time on the mechanical behavior of the common carotid artery from adult mice. Each artery was tested after storage for 1–28 days in physiologic saline at 4°C. There were no significant effects of storage time on the arterial diameter or force at each pressure, but there were significant effects on the stretch ratio and stress at each pressure. The significant effects on the stretch ratio and stress were due to decreases in the unloaded dimensions with storage time, when measured from cut arterial rings. When the unloaded dimensions were measured instead from histology sections, there were no significant changes with storage time. We conclude that histology sections yield a more consistent measurement of the unloaded dimensions and that there are no significant changes in the mechanical behavior of mouse carotid artery with storage up to 28 days.

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Copyright © 2011 by American Society of Mechanical Engineers
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Figures

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Figure 1

Unloaded dimensions from cut ring measurements. Outer diameter and thickness decrease with time, while the inner diameter does not change with time. N1  = 12, N3  = 11, N7  = N14  = N28 = 9 (N and i are the number of samples and the time-point, respectively). ♦ outer diameter, ▪ inner diameter, Δ thickness.

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Figure 2

Effects of pressure and storage time on (a) outer diameter, (b) longitudinal force, and (c) compliance. There is no consistent trend for changes in the curves with storage time. N1  = 12, N3  = 11, N7  = N14  = N28  = 9.

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Figure 3

Effects of pressure and storage time on (a) circumferential stretch ratio, (b) circumferential stress, and (c) longitudinal stress. There is no consistent trend for changes in the curves with storage time. N1  = 12, N3  = 11, N7  = N14  = N28  = 9.

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Figure 4

Circumferential stress-stretch relationship at different storage times. The local slope of the curves gives an incremental measure of the elastic modulus of the arterial wall. Although there are some variations from one time point to the other, there is no consistent trend for changes in the curves with storage time. N1  = 12, N3  = 11, N7  = N14  = N28  = 9.

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Figure 5

Images used for dimension and component measurements: (a) representative cut arterial ring with the inner and outer diameter outlined, (b) VVG stained histology section which clearly shows the boundaries of the media and adventitia in the artery wall, and (c) VVG stained histology image thresholded to highlight the elastic lamellae for area fraction measurements

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Figure 6

Unloaded dimensions obtained from histology sections. Unlike the measurements from cut arterial rings in Fig. 1, there are no consistent trends for changes in the dimensions with storage time. N1  = N3  = 7, N7  = N14  = N28  = 6.

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Figure 7

Fractional area of the elastic lamellae (Ae ) [shown in Fig. 5c] compared to the total in medial area (Am ) in the carotid artery at different storage time points. There are no significant differences between any time points.

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