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Technical Briefs

Effect of Pore Architecture on Oxygen Diffusion in 3D Scaffolds for Tissue Engineering

[+] Author and Article Information
Geunseon Ahn, Jeong Hun Park, Taeyun Kang

Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja dong, Nam-gu, Pohang, Kyeongbuk 790-784, South Korea

Jin Woo Lee

Department of Mechanical Engineering, University of Texas, Austin, TX 78712-0292

Hyun-Wook Kang

Wake Forest Institute for Regenerative Medicine, Wake Forest University, Medical Center Boulevard, Winston-Salem, NC 27157

Dong-Woo Cho1

Department of Mechanical Engineering and Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), San 31, Hyoja dong, Nam-gu, Pohang, Kyeongbuk 790-784, South Koreadwcho@postech.ac.kr

1

Corresponding author.

J Biomech Eng 132(10), 104506 (Sep 28, 2010) (5 pages) doi:10.1115/1.4002429 History: Received April 07, 2010; Revised August 03, 2010; Posted August 24, 2010; Published September 28, 2010; Online September 28, 2010

The aim of this study was to maximize oxygen diffusion within a three-dimensional scaffold in order to improve cell viability and proliferation. To evaluate the effect of pore architecture on oxygen diffusion, we designed a regular channel shape with uniform diameter, referred to as cylinder shaped, and a new channel shape with a channel diameter gradient, referred to as cone shaped. A numerical analysis predicted higher oxygen concentration in the cone-shaped channels than in the cylinder-shaped channels, throughout the scaffold. To confirm these numerical results, we examined cell proliferation and viability in 2D constructs and 3D scaffolds. Cell culture experiments revealed that cell proliferation and viability were superior in the constructs and scaffolds with cone-shaped channels.

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Copyright © 2010 by American Society of Mechanical Engineers
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Figures

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Figure 1

Channel shapes designed for numerical analyses: (a) cylinder shaped and (b) cone shaped

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Figure 2

Computational domains and boundary conditions: (a) cylinder shaped and (b) cone shaped. S1 represents the lumen region and S2 represents the tissue region.

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Figure 3

Oxygen concentration in different pore architectures in static culture

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Figure 4

SEM images of 3D scaffolds: ((a) and (b) cylinder shaped and ((c) and (d)) cone-shaped

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Figure 5

Fluorescence microscopy of cell viability in (a) 2D constructs at days 1 and 7 and (b) 3D scaffolds at day 21. Green indicates live cells, and red indicates dead cells. Magnification, 100× for all images.

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Figure 6

Proliferation of MC3T3-E1 cells in (a) 2D constructs and (b) 3D scaffolds. Experimental data are expressed as mean±standard deviation (n=3; p∗<0.01).

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