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Design Innovation

Metallic Foil-Assisted Laser Cell Printing

[+] Author and Article Information
Yafu Lin

Department of Mechanical Engineering, Clemson University, Clemson, SC 29634

Yong Huang1

Department of Mechanical Engineering, Clemson University, Clemson, SC 29634yongh@clemson.edu

Douglas B. Chrisey

Department of Materials Science and Engineering, and Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180

1

Corresponding author.

J Biomech Eng 133(2), 025001 (Jan 05, 2011) (5 pages) doi:10.1115/1.4003132 History: Received September 17, 2010; Revised November 16, 2010; Posted November 29, 2010; Published January 05, 2011; Online January 05, 2011

Laser direct-write technology such as modified laser-induced forward transfer (LIFT) is emerging as a revolutionary technology for biological construct fabrication. While many modified LIFT-based cell direct writing successes have been achieved, possible process-induced cell injury and death is still a big hurdle for modified LIFT-based cell direct writing to be a viable technology. The objective of this study is to propose metallic foil-assisted LIFT using a four-layer structure to achieve better droplet size control and increase cell viability in direct writing of human colon cancer cells (HT-29). The proposed four layers include a quartz disk, a sacrificial and adhesive layer, a metallic foil, and a cell suspension layer. The bubble formation-induced stress wave is responsible for droplet formation. It is found that the proposed metallic foil-assisted LIFT approach is an effective cell direct-write technology and provides better printing resolution and high post-transfer cell viability when compared with other conventional modified LIFT technologies such as matrix-assisted pulsed-laser evaporation direct-write; at the same time, the possible contamination from the laser energy absorbing material is minimized using a metallic foil.

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Copyright © 2011 by American Society of Mechanical Engineers
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Figures

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Figure 1

Experimental apparatus and schematic of proposed metallic foil-assisted laser cell transfer

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Figure 2

Metallic foil after cell transfer under laser fluence 5720±48 mJ/cm2

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Figure 3

Transferred cell number per laser pulse as a function of laser fluence

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Figure 4

Comparison of post-transfer HT-29 cell viability using MAPLE DW (3) and proposed approach (both with control considered)

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Figure 5

Droplet diameter comparison using MAPLE DW (3) and the proposed approach

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