Research Papers

Micro-Brillouin Scattering Measurements in Mature and Newly Formed Bone Tissue Surrounding an Implant

[+] Author and Article Information
Vincent Mathieu

Laboratoire de Biomécanique Biomatériau Ostéo Articulaire, CNRS,  Université Paris 7, UMR CNRS 7052, 10 Avenue de Verdun, Paris 75010, France

Kenji Fukui, Mami Matsukawa, Masahiko Kawabe

Laboratory of Ultrasonic Electronics, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan

Romain Vayron

Laboratoire de Modélisation et de Simulation Multi-Echelle, UMR CNRS 8208, CNRS,  Université Paris Est, 61 Avenue du Général de Gaulle, Créteil 94010, France

Emmanuel Soffer, Fani Anagnostou

Laboratoire de Biomécanique Biomatériau Ostéo Articulaire and Department of Periodontology, Service of Odontology, Pitié Salpetrière Hospital, et Hôtel-Dieu Hospital AP-HP, U.F.R. of Odontology, Université Paris 7, 5 rue Garancière, 75006 Paris, France

Guillaume Haiat1

Laboratoire de Modélisation et de Simulation Multi-Echelle, UMR CNRS 8208, CNRS,  Université Paris Est, 61 Avenue du Général de Gaulle, Créteil 94010, Francehaiat@u-pec.fr


Corresponding author.

J Biomech Eng 133(2), 021006 (Jan 24, 2011) (6 pages) doi:10.1115/1.4003131 History: Received August 31, 2010; Revised October 29, 2010; Posted November 29, 2010; Published January 24, 2011; Online January 24, 2011

The evolution of implant stability in bone tissue remains difficult to assess because remodeling phenomena at the bone-implant interface are still poorly understood. The characterization of the biomechanical properties of newly formed bone tissue in the vicinity of implants at the microscopic scale is of importance in order to better understand the osseointegration process. The objective of this study is to investigate the potentiality of micro-Brillouin scattering techniques to differentiate mature and newly formed bone elastic properties following a multimodality approach using histological analysis. Coin-shaped Ti–6Al–4V implants were placed in vivo at a distance of 200μm from rabbit tibia leveled cortical bone surface, leading to an initially empty cavity of 200μm×4.4mm. After 7 weeks of implantation, the bone samples were removed, fixed, dehydrated, embedded in methyl methacrylate, and sliced into 190μm thick sections. Ultrasonic velocity measurements were performed using a micro-Brillouin scattering device within regions of interest (ROIs) of 10μm diameter. The ROIs were located in newly formed bone tissue (within the 200μm gap) and in mature bone tissue (in the cortical layer of the bone sample). The same section was then stained for histological analysis of the mineral content of the bone sample. The mean values of the ultrasonic velocities were equal to 4.97×103m/s in newly formed bone tissue and 5.31×103m/s in mature bone. Analysis of variance (p=2.42×104) tests revealed significant differences between the two groups of measurements. The standard deviation of the velocities was significantly higher in newly formed bone than in mature bone. Histological observations allow to confirm the accurate locations of the velocity measurements and showed a lower degree of mineralization in newly formed bone than in the mature cortical bone. The higher ultrasonic velocity measured in newly formed bone tissue compared with mature bone might be explained by the higher mineral content in mature bone, which was confirmed by histology. The heterogeneity of biomechanical properties of newly formed bone at the micrometer scale may explain the higher standard deviation of velocity measurements in newly formed bone compared with mature bone. The results demonstrate the feasibility of micro-Brillouin scattering technique to investigate the elastic properties of newly formed bone tissue.

Copyright © 2011 by American Society of Mechanical Engineers
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Figure 1

Coin-shaped implant with its surrounding cap

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Figure 2

Schematic representation of the coin-shaped implant model

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Figure 3

Schematic representation of the RIΘA Brillouin scattering geometry. IB corresponds to the incident beam from laser; SB to the scattered beam; Θ/2 to the outer scattering angle between the normal to the sample and SB; kI to the incident photon wave vector; kS to the scattered wave vector and kB to the measured phonon wave vector.

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Figure 4

Image of the specimen specifying the locations of the 12 volumes measured with micro-Brillouin scattering. Regions 1–6 are located in newly formed bone tissue and regions 7–12 in mature bone tissue.

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Figure 5

Image taken after histological preparation. Crimson stain shows the degree of mineralization of bone and allows to discriminate newly formed bone tissue (less mineralized, clear crimson) from mature bone (more mineralized, dark crimson).




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