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TECHNICAL PAPERS: Fluids/Heat/Transport

Numerical Simulation on Mass Transport in a Microchannel Bioreactor for Co-culture Applications

[+] Author and Article Information
Yan Zeng, Thong-See Lee, Peng Yu

Department of Mechanical Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576

Hong-Tong Low1

Division of Bioengineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576mpelowht@nus.edu.sg

1

Corresponding author.

J Biomech Eng 129(3), 365-373 (Oct 18, 2006) (9 pages) doi:10.1115/1.2720913 History: Received March 31, 2006; Revised October 18, 2006

Microchannel bioreactors have applications for manipulating and investigating the fluid microenvironment on cell growth and functions in either single culture or co-culture. This study considers two different types of cells distributed randomly as a co-culture at the base of a microchannel bioreactor: absorption cells, which only consume species based on the Michaelis-Menten process, and release cells, which secrete species, assuming zeroth order reaction, to support the absorption cells. The species concentrations at the co-culture cell base are computed from a three-dimensional numerical flow-model incorporating mass transport. Combined dimensionless parameters are proposed for the co-culture system, developed from a simplified analysis under the condition of decreasing axial-concentration. The numerical results of species concentration at the co-culture cell-base are approximately correlated by the combined parameters under the condition of positive flux-parameter. Based on the correlated results, the critical value of the inlet concentration is determined, which depends on the effective microchannel length. For the flow to develop to the critical inlet concentration, an upstream length consisting only of release cells is needed; this upstream length is determined from an analytical solution. The generalized results may find applications in analyzing the mass transport requirements in a co-culture microchannel bioreactor.

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Copyright © 2007 by American Society of Mechanical Engineers
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Figures

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Figure 6

Co-culture concentration-reaction parameter at base for condition of positive flux parameter Daaβ>0: (a) at different β and constant Daa (b) at different Daa and constant β

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Figure 7

Critical inlet concentrations for effective length ℓ∕hPe at various flux-parameter Daaβ

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Figure 8

Designed upstream length to achieve a critical inlet concentration

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Figure 1

Scheme of the microchannel bioreactor (not to scale)

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Figure 2

Validation of numerical model (from Ref. 12)

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Figure 3

Species concentration profile in the co-culture microchannel of aspect ratio α=0.4: (i) axial vertical plane, (ii) bottom plane, and (iii) transverse vertical plane. Parameters are Pe=100, Daa=0.5, K¯ma=0.068. (a) αDa=1.5 (increasing axial-concentration) and (b) αDa=0.5 (decreasing axial-concentration).

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Figure 4

Nondimensional concentration distribution at base at different αDa at Pe=10, K¯ma=0.068, with Daa=0.1 and 0.5: (a) αDa⩾1∕(1+K¯ma) (increasing or constant axial-concentration) and (b) αDa<1∕(1+K¯ma) (decreasing axial-concentration)

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Figure 5

Co-culture concentration-reaction parameter at base for condition of negative flux parameter Daaβ<0: (a) at different β and constant Daa and (b) at different Daa and constant β

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