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TECHNICAL PAPERS: Soft Tissue

Frictional Response of Bovine Articular Cartilage Under Creep Loading Following Proteoglycan Digestion With Chondroitinase ABC

[+] Author and Article Information
Ines M. Basalo

Department of Mechanical Engineering, Columbia University, New York, NY 10027

Faye Hui Chen

Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20982

Clark T. Hung

Department of Biomedical Engineering, Columbia University, New York, NY 10027

Gerard A. Ateshian1

Departments of Mechanical Engineering and Biomedical Engineering, Columbia University, New York, NY 10027ateshian@columbia.edu

1

To whom correspondence should be addressed.

J Biomech Eng 128(1), 131-134 (Sep 06, 2005) (4 pages) doi:10.1115/1.2133764 History: Received June 06, 2005; Revised September 06, 2005

Abstract

The specific aim of this study was to investigate the effect of chondroitinase ABC treatment on the frictional response of bovine articular cartilage against glass, under creep loading. The hypothesis is that chondroitinase ABC treatment increases the friction coefficient of bovine articular cartilage under creep. Articular cartilage samples $(n=12)$ harvested from two bovine knee joints ($1–3months$ old) were divided into a control group (intact specimens) and a treated group (chondroitinase ABC digestion), and tested in unconfined compression with simultaneous continuous sliding ($±4mm$ at $1mm∕s$) under a constant applied stress of $0.5MPa$, for $2500s$. The time-dependent response of the friction coefficient was measured. With increasing duration of loading, treated samples exhibited a significantly higher friction coefficient than control samples as assessed by the equilibrium value (treated: $μeq=0.19±0.02$; control: $μeq=0.12±0.03$; $p=0.002$), though the coefficient achieved immediately upon loading did not increase significantly (treated: $μmin=0.0053±0.0025$; control: $μmin=0.037±0.0013$; $p=0.19$). Our results demonstrate that removal of the cartilage glycosaminoglycans using chondroitinase ABC significantly increases the overall time-dependent friction coefficient of articular cartilage. These findings strengthen the motivation for developing chondroprotective strategies by increasing cartilage chondroitin sulfate content in osteoarthritic joints.

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Figures

Figure 1

Average and standard deviation of the time-dependent friction coefficient μeff for the chondroitinase treated (black line) and control (gray line) groups (n=6 per group)

Figure 2

Average and standard deviation of the response of the creep strain for the chondroitinase treated and control groups (n=6 per group)

Figure 3

Average friction coefficient obtained under a stress-relaxation testing configuration (10% compressive strain after an application of a tare load of 1.8±0.4N), from the study of Basalo (see Ref. 9)

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