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TECHNICAL PAPERS: Cell

Correlation of HSP70 Expression and Cell Viability Following Thermal Stimulation of Bovine Aortic Endothelial Cells

[+] Author and Article Information
Marissa Nichole Rylander

 The University of Texas at Austin, Biomedical Engineering, 1 University Station C0800, Austin, TX 78712-0238n.forney@mail.utexas.edu

Kenneth R. Diller

 The University of Texas at Austin, Biomedical Engineering, 1 University Station C0800, Austin, TX 78712-0238kdiller@mail.utexas.edu

Sihong Wang

 Harvard Medical School, Research Department, Rm. 249, Shriners Hospitals for Children, 51 Blossom Street, Boston, MA 02114sihong̱wang@hms.harvard.edu

Shanti J. Aggarwal

 The University of Texas at Austin, Biomedical Engineering, 1 University Station C0800, Austin, TX 78712-0238sja@mail.utexas.edu

J Biomech Eng 127(5), 751-757 (May 31, 2005) (7 pages) doi:10.1115/1.1993661 History: Received April 01, 2004; Revised May 31, 2005

Thermal preconditioning protocols for cardiac cells were identified which produce elevated HSP70 levels while maintaining high cell viability. Bovine aortic endothelial cells were heated with a water bath at temperatures ranging from 44to50°C for periods of 130min. Thermal stimulation protocols were determined which induce HSP70 expression levels ranging from 2.3 to 3.6 times the control while maintaining cell viabilities greater than 90%. An Arrhenius injury model fit to the cell damage data yielded values of A=1.4×1066s1 and Ea=4.1×105Jmol. Knowledge of the injury parameters and HSP70 kinetics will enhance dosimetry guideline development for thermal stimulation of heat shock proteins expression in cardiac tissue.

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Copyright © 2005 by American Society of Mechanical Engineers
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Figures

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Figure 1

Temperature measured within medium and flask wall

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Figure 2

Normalized HSP70/actin as a function of heating time and temperature evaluated at 16–18h postheating with an average error of ±0.13mg∕ml, n=3

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Figure 3

Cell viability following variable duration of thermal stress for T=44(a),46(b),48(c),50(d) °C measured at t=1,24,48,72h postheating with an average error of ±3%, n=3.

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Figure 4

Cell viability measured 72h postheating and HSP70/actin concentration determined 16h postheating as a function of thermal stimulation duration for T=44(a),46(b),48(c),50(d) °C, n=3.

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Figure 5

Best linear fit to natural logarithms of threshold times of thermal damage vs 1∕T for 72h postheating, n=3

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Figure 6

Comparison between measured and Arrhenius model-predicted damage for 72h postheating, n=3

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