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TECHNICAL BRIEFS

Effect of Mercury Column on the Microdynamics of the Piezo-Driven Pipettes

[+] Author and Article Information
Kerem Ediz

Mechanical Engineering Department,  University of Connecticut, Storrs, CT 06269-3139

Nejat Olgac

Mechanical Engineering Department,  University of Connecticut, Storrs, CT 06269-3139olgac@engr.uconn.edu

J Biomech Eng 127(3), 531-535 (Jan 31, 2005) (5 pages) doi:10.1115/1.1894368 History: Received August 04, 2003; Revised October 07, 2004; Accepted January 31, 2005

This study is on an interesting phenomenon concerning cellular microinjection procedures which are used for various biomedical applications, and in particular intracytoplasmic sperm injection. Recent years have brought considerable practical improvements in these operations. One of them suggests aspirating a very small quantity of mercury in the injection pipettes prior to piercing into cells. This process is proven to enhance the rate of success considerably. We present a unique study in determining the influence of mercury on the microdynamics of the pipette. The effort contains both numerical simulations and corresponding experimental verification. Ultimately we offer two critical results: (1) The mercury column increases the mass loading and expectedly decreases the natural frequencies of the pipette and (2) The lateral oscillations, which play a destructive role in piercing, are subdued in amplitude due to the mass loading of mercury. Simulation results are presented, which are also verified experimentally using high-speed digital imaging. As a consequence of these findings we also propose some alternative design directions for future microinjection devices.

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Copyright © 2005 by American Society of Mechanical Engineers
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Figures

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Figure 2

Injection pipette

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Figure 3

Components in the process

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Figure 4

Description of the segments in the microinjector

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Figure 5

Waterfall depiction of the damped pipette oscillations with mercury

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Figure 6

Waterfall depiction of the damped pipette oscillations without mercury

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Figure 7

Experimental setup for piezoassisted ICSI for mouse

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Figure 8

Procedure of the experiment

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Figure 9

(a) Dimensions of the drawn section (b) deformed shape of the drawn section without mercury

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Figure 10

(a) Dimensions of the drawn section with mercury (dark) (b) deformed shape of the drawn section with mercury

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