A Multiaxial Computer-Controlled Organ Culture and Biomechanical Device for Mouse Carotid Arteries

[+] Author and Article Information
R. L. Gleason, S. P. Gray

Department of Biomedical Engineering, Texas A&M University, College Station, TX

E. Wilson

Department of Medical Physiology and Cardiovascular Research Institute, Texas A&M University System Health Science Center, College Station, TX

J. D. Humphrey

Department of Biomedical Engineering and M.E. DeBakey Institute, Texas A&M University, College Station, TX

J Biomech Eng 126(6), 787-795 (Feb 04, 2005) (9 pages) doi:10.1115/1.1824130 History: Received December 17, 2003; Revised June 08, 2004; Online February 04, 2005
Copyright © 2004 by ASME
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Plan view of the overall organ culture and biomechanical testing device. See Table 1 for a detailed parts list.
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Profile view of incubator chamber and mounting plate. See Table 1 for a detailed parts list.
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Imaging of an isolated mouse common carotid artery with three 20-μm diameter video-tracking microspheres placed along the axis, which can be tracked in real time to monitor the local in-plane stretches. Here, Pv=93 mmHg,λz=1.5, and Q=0.75 mL/min. Note that the vessel is translucent and the dark spot resulted from loose adventitia on the rear surface. The translucent character allows inner and outer diameter to be measured optically.
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(a) Pressure waveforms at various locations along the luminal flow loop. Notice that the vessel pressure, approximated as Pv=P2+kΔPv (where ΔPv=P1−P2), ranges from 80 to 120 mmHg as desired. (b) Differential pressure waveforms ΔPv and ΔPf(=P2−P3). (c) Axial load response to pulsatile pressure in (a).
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Illustration of independent control of pressure, flow, and axial load. (a) Step changes in flow while maintaining constant pressure and axial load. (b) Step changes in pressure while maintaining constant flow and axial load. (c) Step changes in axial load while maintaining constant pressure and flow.
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(a) Pressure–diameter and pressure–axial force curves during cyclic pressurization tests at fixed lengths on a freshly isolated mouse common carotid artery. Note the commonly observed force responses.
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(a) Pressure–diameter data from cyclic pressurization tests and (b) axial force–length data from cyclic extension tests at days 0, 1, 2, 3, and 4 during culture at Pv=100±20 mmHg (5 Hz), Q=0.50 mL/min, and λz*=1.80.
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Mean Cauchy stress–stretch data for (a) cyclic pressurization and (b) cyclic extension tests at multiple fixed axial stretches and luminal pressures, respectively. Data are from a control (day 0) vessel, with mean volume V̄=0.270 mm3, and unloaded length L=5.38 mm, diameter D=369 μm, thickness H=50 μm, and H:Rmid=0.31.
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Typical response to subsequent administration of 10−5 M phenylehprine, 10−5 M acetylcholine, and 10−4 M sodium nitropruside in (a) freshly isolated and (b) 4-day cultured vessels. Note: vessel cultured at Pv=60 mmHg (steady), Q=0.75 mL/min,λz=1.65.



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