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TECHNICAL PAPERS: Soft Tissue

A Multiaxial Computer-Controlled Organ Culture and Biomechanical Device for Mouse Carotid Arteries

[+] Author and Article Information
R. L. Gleason, S. P. Gray

Department of Biomedical Engineering, Texas A&M University, College Station, TX

E. Wilson

Department of Medical Physiology and Cardiovascular Research Institute, Texas A&M University System Health Science Center, College Station, TX

J. D. Humphrey

Department of Biomedical Engineering and M.E. DeBakey Institute, Texas A&M University, College Station, TX

J Biomech Eng 126(6), 787-795 (Feb 04, 2005) (9 pages) doi:10.1115/1.1824130 History: Received December 17, 2003; Revised June 08, 2004; Online February 04, 2005
Copyright © 2004 by ASME
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References

Bardy,  N., Karillon,  G. J., Merval,  R., Samuel,  J.-L., and Tedgui,  A., 1995, “Differential Effects of Pressure and Flow on DNA and Protein Synthesis and on Fibronectin Expression by Arteries in a Novel Organ Culture System,” Circ. Res., 77, pp. 684–694.
Labadie,  R. F., Antaki,  J. F., Williams,  J. L., Katyal,  S., Ligush,  J., Watkins,  S. C., Pham,  S. M., and Borovetz,  H. S., 1996, “Pulsatile Perfusion System for Ex Vivo Investigation of Biochemical Pathways in Intact Vascular Tissue,” Am. J. Physiol., 270(Heart Circ. Physiol. 39 pp. ), H760–H768.
Chestler,  N. C., Conklin,  B. S., Han,  H.-C., and Ku,  D. K., 1998, “Simplified Ex Vivo Artery Culture Techniques for Porcine Arteries,” Annu. Rep. Prog. Chem., Sect. C: Phys. Chem., 4, pp. 123–127.
Matsumoto,  T., Okumura,  E., Miura,  Y., and Sato,  M., 1999, “Mechanical and Dimensional Adaptation of Rabbit Carotid Artery In Vitro,” Med. Biol. Eng. Comput., 37, pp. 252–256.
Bakker,  E. N. T. P., van der Meulen,  E. T., Spaan,  J. A. E., and VanBavel,  E., 2000, “Organoid Culture of Cannulated Rat Resistance Arteries: Effect of Serum Factors on Vasoactivity and Remodeling,” Am. J. Physiol., 278, pp. H1233–H1240.
Bolz,  S.-S., Pieperhoff,  S., de Wit,  C., and Pohl,  U., 2000, “Intact Endothelial and Smooth Muscle Function in Small Resistance Arteries after 48 h in Vessel Culture,” Am. J. Physiol., 279, pp. H1434–H1439.
Clerin,  V., Nichol,  J. W., Petko,  M., Myung,  R. J., Gaynor,  J. W., and Gooch,  K. J., 2003, “Tissue Engineering of Arteries by Direct Remodeling of Intact Arterial Segments,” Tissue Eng., 9, pp. 461–472.
Humphrey,  J. D., Kang,  T., Sakarda,  P., and Anjanappa,  M., 1993, “Computer-aided Vascular Experimentation: A New Electromechanical Test System,” Ann. Biomed. Eng., 21, pp. 33–43.
Hartley,  C. J., Michael,  L. H., and Enthman,  M. L., 1995, “Noninvasive Measurement of Ascending Aortic Blood Velocity in Mice,” Am. J. Physiol., 268, pp. H499–H505.
Farrehi,  P. M., Ozaki,  C. K., Carmeliet,  P., and Fay,  W. P., 1998, “Regulation of Arterial Thrombolysis by Plasminogen Activator Inhibitor-1 in Mice,” Circulation, 97, pp. 1002–1008.
Transonic Systems, Inc., 1997, “Tools and Techniques for Hemodynamic Studies in Mice,” Available at http://www.transonic.com.
Rudic,  R. D., Bucci,  M., Fulton,  D., Segal,  S. S., and Sessa,  W. C., 2000, “Temporal Events Underlying Arterial Remodeling after Chronic Flow reduction in Mice. Correlation of Structural Changes with a Deficit in Basal Nitric Oxide Synthesis,” Circ. Res., 86, pp. 1160–1166.
Sullivan,  C. J., and Hoying,  J. B., 2002, “Flow-dependent Remodeling in the Carotid Artery of Fibroblast Growth Factor-2 Knockout Mice,” Arterioscler., Thromb., Vasc. Biol., 22(7), pp. 1100–1105.
Gross,  V., and Luft,  F. C., 2003, “Exercising Restraint in Measuring Blood Pressure in Conscious Mice,” Hypertension, 41, pp. 879–881.
Li,  Y.-H., Reddy,  A. K., Taffet,  G. E., Michael,  L. H., Entman,  M. L., and Hartley,  C. J., 2003, “Doppler Evaluation of Peripheral Vascular Adaptations to Transverse Aortic Banding in Mice,” Ultrasound Med. Biol., 29(9), pp. 1281–1289.
Langille,  B. L., Bendeck,  M. L., and Keeley,  F. W., 1989, “Adaptations of Carotid Arteries of Young and Mature Rabbits to Reduced Carotid Blood Flow,” Am. J. Physiol., 256 (Heart Circ. Physiol. 25), pp. H931–H939.
Matsumoto,  T., and Hayashi,  K., 1994, “Mechanical and Dimensional Adaptation of Rat Aorta to Hypertension,” ASME J. Biomech. Eng., 116, pp. 278–283.
Jackson,  Z. S., Gotlieb,  A. I., and Langille,  L., 2002, “Wall Tissue Remodeling Regulates Longitudinal Tension in Arteries,” Circ. Res., 90, pp. 918–925.
Mangiarua,  E. I., Moss,  N., Lemke,  S. M., McCumbee,  W. D., Szarek,  J. J., and Gruetter,  C. A., 1992, “Morphological and Contractile Characteristics of Rat Aortae Perfused for 3 and 6 Days In Vitro,” Artery, 19, pp. 14–38.
Han,  H.-C., and Ku,  D. N., 2001, “Contractile Response in Arteries Subjected to Hypertensive Pressure in Seven-day Organ Culture,” Ann. Biomed. Eng., 29, pp. 467–475.
Kim,  I., Je,  H.-D., Gallant,  C., Zhan,  Q., Va Riper,  D., Badwey,  J. A., Singer,  H. A., and Morgan,  K. G., 2000, “Ca2+-Calmodulin-dependent Protein Kinase II-Dependent Activation of Contractility in Ferret Aorta,” J Physio., 256, 2, pp. 367–374.
Lemarie,  C. A., Esposito,  B., Tedgui,  A., and Lehoux,  S., 2003, “Pressure-induced Vascular Adaptation of Nuclear Factor-κB. Role of Cell Survival,” Circ. Res., 93, pp. 207–212.
Lehoux,  S., Lemarie,  C. A., Esposito,  B., Lijnen,  H. R., and Tedgui,  A., 2004, “Pressure-induced Matrix Metalloproteinase-9 Contributes to Early Hypertension Remodeling,” Circulation, 109, pp. 1041–1047.
Vorp,  D. A., Severyn,  D. A., Steed,  D. L., and Webster,  M. W., 1996, “A Device for the Application of Cyclic Twist and Extension on Perfused Vascular Segments,” Am. J. Physiol., 270 (Heart Circ. Physiol. 39), pp. H787–H795.
Gan,  L., Sjogren,  L. S., Doroudi,  R., and Jern,  S., 1999, “A New Computerized Biomechanical Perfusion Model for Ex Vivo Study of Fluid Mechanical Forces in Intact Conduit Vessels,” J. Vasc. Res., 36, pp. 68–78.
Faury,  G., Maher,  G. M., Li,  D. Y., Keating,  M. T., Mecham,  R. P., and Boyle,  W. A., 1999, “Relation between Outer and Luminal Diameter in Cannulated Arteries,” Am. J. Physiol., 277 (Heart Circ. Physiol. 46), pp. H1745–H1753.
Faury,  G., Pezet,  M., Knutsen,  R. H., Boyle,  W. A., Heximer,  S. P., McLean,  S. E., Minkes,  R. E., Blumer,  K. L., Kovacs,  A., Kelly,  D. P., Li,  D. Y., Starcher,  B., and Mecham,  R. P., 2003, “Developmental Adaptation of the Mouse Cardiovascular System to Elastin Haploinsufficiency,” J. Clin. Invest., 112, pp. 1419–1428.
Niklason,  L. E., Gao,  J., Abbot,  J. M., Hirschi,  K. K., Houser,  S., Marini,  R., and Langer,  R., 1999, “Functional Arteries Grown In Vitro” Science, 284, pp. 489–493.
Moore,  J. E., Burki,  E., Suciu,  A., Zhao,  S., Burnier,  M., Brunner,  H. R., and Meister,  J.-J., 1994, “A Device for Subjection Vascular Endothelial Cells to both Fluid Shear Stress and Circumferential Cyclic Stretch,” Ann. Biomed. Eng., 22, pp. 416–422.
Brant,  A. M., Chmielewski,  J. F., Hung,  T.-K., and Borovetz,  H. S., 1986, “Simulation In Vitro of Pulsatile Vascular Hemodynamics using a CAD/CAM-designed Cam Disc and Roller Follower,” Artif. Organs, 10, pp. 419–421.
Taber,  L. A., 1998, “A Model of Aortic Growth based on Fluid Shear and Fiber Stresses,” ASME J. Biomech. Eng., 120, pp. 348–354.
Rachev,  A., 2000, “A Model of Arterial Adaptation to Alterations in blood flow,” J. Elast., 61, pp. 83–111.
Gleason,  R. L., Taber,  L. A., and Humphrey,  J. D., 2004, “A 2-D Model of Flow-induced Alterations in the Geometry, Structure, and Properties of Carotid Arteries,” ASME J. Biomech. Eng., 126 pp. 371–381.
Humphrey, J. D., 2002, Cardiovascular Solid Mechanics: Cells, Tissues, and Organs, Springer, New York.

Figures

Grahic Jump Location
(a) Pressure–diameter data from cyclic pressurization tests and (b) axial force–length data from cyclic extension tests at days 0, 1, 2, 3, and 4 during culture at Pv=100±20 mmHg (5 Hz), Q=0.50 mL/min, and λz*=1.80.
Grahic Jump Location
Mean Cauchy stress–stretch data for (a) cyclic pressurization and (b) cyclic extension tests at multiple fixed axial stretches and luminal pressures, respectively. Data are from a control (day 0) vessel, with mean volume V̄=0.270 mm3, and unloaded length L=5.38 mm, diameter D=369 μm, thickness H=50 μm, and H:Rmid=0.31.
Grahic Jump Location
Typical response to subsequent administration of 10−5 M phenylehprine, 10−5 M acetylcholine, and 10−4 M sodium nitropruside in (a) freshly isolated and (b) 4-day cultured vessels. Note: vessel cultured at Pv=60 mmHg (steady), Q=0.75 mL/min,λz=1.65.
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Profile view of incubator chamber and mounting plate. See Table 1 for a detailed parts list.
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Plan view of the overall organ culture and biomechanical testing device. See Table 1 for a detailed parts list.
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Imaging of an isolated mouse common carotid artery with three 20-μm diameter video-tracking microspheres placed along the axis, which can be tracked in real time to monitor the local in-plane stretches. Here, Pv=93 mmHg,λz=1.5, and Q=0.75 mL/min. Note that the vessel is translucent and the dark spot resulted from loose adventitia on the rear surface. The translucent character allows inner and outer diameter to be measured optically.
Grahic Jump Location
(a) Pressure waveforms at various locations along the luminal flow loop. Notice that the vessel pressure, approximated as Pv=P2+kΔPv (where ΔPv=P1−P2), ranges from 80 to 120 mmHg as desired. (b) Differential pressure waveforms ΔPv and ΔPf(=P2−P3). (c) Axial load response to pulsatile pressure in (a).
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Illustration of independent control of pressure, flow, and axial load. (a) Step changes in flow while maintaining constant pressure and axial load. (b) Step changes in pressure while maintaining constant flow and axial load. (c) Step changes in axial load while maintaining constant pressure and flow.
Grahic Jump Location
(a) Pressure–diameter and pressure–axial force curves during cyclic pressurization tests at fixed lengths on a freshly isolated mouse common carotid artery. Note the commonly observed force responses.

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