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TECHNICAL PAPERS: Soft Tissue

Cartilage Interstitial Fluid Load Support in Unconfined Compression Following Enzymatic Digestion

[+] Author and Article Information
Ines M. Basalo

Department of Mechanical Engineering, Columbia University, New York, NY 10027

Robert L. Mauck, Terri-Ann N. Kelly, Clark T. Hung

Department of Biomedical Engineering, Columbia University, New York, NY 10027

Steven B. Nicoll

Department of Bioengineering, University of Pennsylvania

Faye H. Chen

Department of Orthopedic Surgery, Columbia University, New York, NY 10032

Gerard A. Ateshian

Departments of Mechanical Engineering and Biomedical Engineering, Columbia University, New York, NY 10027

J Biomech Eng 126(6), 779-786 (Feb 04, 2005) (8 pages) doi:10.1115/1.1824123 History: Received September 23, 2003; Revised July 26, 2004; Online February 04, 2005
Copyright © 2004 by ASME
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References

Figures

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Diagram of the unconfined compression testing chamber
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Typical response of (a) W(t) and Wp(t) and (b) plot of Wp(t) versus W(t) during the loading phase before enzymatic treatment
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Typical response of (a) W(t) and Wp(t) and (b) plot of Wp(t) versus W(t) during the loading phase after treatment with chondroitinase ABC
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Summary of peak fluid load support for loaded treatment groups and their respective controls: (a) Collagenase groups; (b) chondroitinase ABC groups
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Histological sections stained for type II collagen (magnification 10X) for (a) a PBS loaded control specimen, and specimens treated with (b) 0.7 u/mg wet-weight and (c) 1.0 u/mg wet-weight of collagenase (bar=100 μm)

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