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TECHNICAL PAPERS: Cell

Local, Three-Dimensional Strain Measurements Within Largely Deformed Extracellular Matrix Constructs

[+] Author and Article Information
Blayne A. Roeder, Klod Kokini

Department of Biomedical Engineering, Purdue University, 500 Central Drive, West Lafayette, IN 47907-2022 School of Mechanical Engineering, Purdue University, 585 Purdue Mall, West Lafayette, IN 47907-2088

J. Paul Robinson, Sherry L. Voytik-Harbin

Department of Biomedical Engineering, Purdue University, 500 Central Drive, West Lafayette, IN 47907-2022, USAand Department of Basic Medical Sciences, Purdue University, 625 Harrison Street, West Lafayette, IN 47907-2026

J Biomech Eng 126(6), 699-708 (Feb 04, 2005) (10 pages) doi:10.1115/1.1824127 History: Received January 06, 2004; Revised June 10, 2004; Online February 04, 2005
Copyright © 2004 by ASME
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Figures

Grahic Jump Location
The effect of filtering on displacement measurement precision (standard deviation) as a function of subvolume size. Correlation values obtained for individual subvolumes were selectively removed based upon defined signal to noise ratio.
Grahic Jump Location
A series of 3D confocal reflection images (shown as 2D projections) representing a correlated subvolume within a collagen ECM during incremental mechanical loading as tracked by the IVDC algorithm
Grahic Jump Location
Progressive volumetric deformation and changes in the microlevel strain distribution for a region within a mechanically loaded collagen ECM. Maximum principle strain (extensional stretch) is plotted on the contour for applied macro level strains of (a) 0; (b) 0.1; (c) 0.2; and (d) 0.3. Original image represents a 100×100×25 μm3 volume within the collagen ECM.
Grahic Jump Location
Changes in the six independent components of the Lagrangian strain (means over the entire volume evaluated) as the external mechanical load was incrementally increased. Error bars indicate standard deviation.

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