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TECHNICAL PAPERS: Cell

Quantitative Examination of a Perfusion Microscope for the Study of Osmotic Response of Cells

[+] Author and Article Information
Hiroshi Takamatsu

Department of Mechanical Engineering Science, Kyushu University 6-10-1, Hakozaki, Higashi-ku Fukuoka 812-8581, Japan Phone: +81-92-642-3471 Fax: +81-92-642-3471e-mail: takamatsu@mech.kyushu-u.ac.jp

Yuichi Komori

Mazda Motor Corp., 3-1, Shinchi, Fuchu-cho, Aki-gun, Hiroshima 730-8670, Japan Phone: +81-82-287-4323 e-mail: komori.y@mazda.co.jp

Sylwia Zawlodzka

Interdisciplinary Graduate School of Engineering Sciences, Kyushu University, 6-1 Kasugakoen, Kasuga 816-8580, Japan Phone: +81-92-583-7882e-mail: sylwia@cm.kyushu-u.ac.jp

Motoo Fujii

Institute for Materials Chemistry and Engineering, Kyushu University, 6-1 Kasugakoen, Kasuga 816-8580, Japan Phone: +81-92-583-7823e-mail: fujii@cm.kyushu-u.ac.jp

J Biomech Eng 126(4), 402-409 (Sep 27, 2004) (8 pages) doi:10.1115/1.1784474 History: Received August 04, 2003; Revised November 23, 2003; Online September 27, 2004
Copyright © 2004 by ASME
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References

Figures

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Schematic showing the structure of the perfusion chamber mounted on the microscope stage
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Schematic of the perfusion microscope system
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Schematic of the laser interferometer for the measurement of NaCl concentration change in the perfusion chamber
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Interference fringe patterns during perfusion of the uniform isotonic solution (a), and the pattern during change in NaCl concentration (b)
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Movement of fringes at the cross line in the middle of the perfusion chamber
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Irradiance at three adjacent pixels as a function of time for the abrupt change in concentration from 0.15 M to 3.8 M NaCl solution
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Normalized concentration at five locations on a cross line for the abrupt increase and gradual increase at 5 M/min from 0.15 M to 3.8 M NaCl solution
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Normalized concentration obtained by replicated experiments for the abrupt increase and gradual increase at 5 M/min from 0.15 M to 3.8 M NaCl solution. The data are the average of the concentration at five locations on a cross line at 0.5-mm intervals.
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Actual concentration change in the perfusion chamber for the abrupt increase and gradual increase at 5 M/min (a), and gradual increase at 0.2 M/min (b)
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Comparison of measured and given rates of concentration change. The measured data are the average of the normalized concentrations ranging between 0.25 and 0.75.
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Cell appearance in the isotonic solution (a), and after exposure to the 0.5 M NaCl solution at 11°C (b).
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Volume change of a cell in response to the concentration change from 0.15 M to 0.5 M NaCl solution at 23°C. The transient concentration change in the extracellular solution, which is based on the measure data, is shown by a solid curve. The normalized cell volume is defined as the cell volume divided by the original cell volume under the isotonic condition. The measured data (symbols) are compared with predicted data (solid curve) with the best fit Lp value and the estimated volume of a cell equilibrated with the extracellular solution (dotted curve). The predicted concentration of the intracellular solution is also shown (dashed curve).
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Cell membrane permeability to water as a function of the reciprocal of temperature. The solid line is the least-squares linear fit of Eq. (14) to the data points.

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