A Molecular Rotor as Viscosity Sensor in Aqueous Colloid Solutions

[+] Author and Article Information
W. Akers, M. A. Haidekker

University of Missouri-Columbia, Department of Biological Engineering, Columbia, MO

J Biomech Eng 126(3), 340-345 (Jun 24, 2004) (6 pages) doi:10.1115/1.1762894 History: Received August 01, 2003; Revised November 17, 2003; Online June 24, 2004
Copyright © 2004 by ASME
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Grahic Jump Location
Molecular structure of 9-[(2-Cyano-2-hydroxy carbonyl)vinyl]julolidine (CCVJ) in relaxed state. Arrow indicates center of rotation.
Grahic Jump Location
Comparison of CCVJ absorbance and emission at different CCVJ concentrations and viscosities modulated by hetastarch content. While emission intensity increases with both CCVJ concentration and viscosity, absorbance increases strongly with concentration only.
Grahic Jump Location
CCVJ fluorescence intensity versus fluid viscosity in solutions of dextran with relatively small molecular size (77.8 kDa), large molecular size (249 kDa) and a mixture: two parts dextran 249 to one part dextran 77.8. Viscosity was measured in a cone-and-plate viscometer at a shear rate of 450 s−1 . The graphs for dextran 77.8 and 249 are combinations of four sample sets each (N=15, 10, 10, and 6). In order to combine them, the y-intercept (the constant C in Eq. (2) was normalized with respect to concentration as determined spectroscopically, thus eliminating concentration fluctuations. A good fit of a straight line in the double-logarithmic scale indicates a power-law relationship between intensity and viscosity over the entire viscosity range. The slopes are 0.72 (r2=0.95;77.8 kDa), 0.49 (r2=0.95;249 kDa) and 0.64 (r2=0.99; mix), respectively. These results also show that viscosity sensitivity is independent of CCVJ concentration.
Grahic Jump Location
Typical CCVJ emission spectra in dextran solutions of 78.8 kDa and 249 kDa molecular weight. No wavelength shift of the emission maximum was found indicating that a change of the environment polarity was negligible.
Grahic Jump Location
CCVJ fluorescence intensity vs. fluid viscosity in solutions of hetastarch and pentastarch. Viscosity was measured in a cone-and-plate viscometer at a shear rate of 450 s−1 . As opposed to dextran, the intensity-viscosity-relationship is biexponential with a higher slope in the low-viscosity range. A very good fit of a straight line can be achieved in the viscosity range from 1.5 mPa s to 6.0 mPa s which yields the exponents (slopes) 0.73 (r2=0.96; hetastarch) and 0.59 (r2=0.97; pentastarch).
Grahic Jump Location
Typical emission spectra of CCVJ in solutions of hetastarch and pentastarch at different viscosities. Similar to dextran, no wavelength shift of the emission maximum can be seen which indicates that the polarity of the environment did not change.




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