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TECHNICAL PAPERS: Soft Tissue

Strain-Rate Sensitive Mechanical Properties of Tendon Fascicles From Mice With Genetically Engineered Alterations in Collagen and Decorin

[+] Author and Article Information
Paul S. Robinson, Tony W. Lin, Paul R. Reynolds, Louis J. Soslowsky

McKay Orthopaedic Research Laboratory, University of Pennsylvania, Philadelphia, PA

Kathleen A. Derwin

Department of Biomedical Engineering and the Orthopaedic Research Center, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH

Renato V. Iozzo

Department of Pathology, Anatomy, and Cell Biology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA

J Biomech Eng 126(2), 252-257 (May 04, 2004) (6 pages) doi:10.1115/1.1695570 History: Received November 08, 2002; Revised August 03, 2003; Online May 04, 2004
Copyright © 2004 by ASME
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References

Woo,  S. L., 1982, “Mechanical Properties of Tendons and Ligaments. I. Quasi-Static and Nonlinear Viscoelastic Properties,” Biorheology, 19(3), pp. 385–96.
Fung,  Y. C., 1968, “Biomechanics: Its Scope, History, and Some Problems of Continuum Mechanics in Physiology,” Appl. Mech. Rev., 21, pp. 1–20.
Pioletti,  D. P., Rakotomanana,  L. R., and Leyvraz,  P. F., 1999, “Strain Rate Effect on the Mechanical Behavior of the Anterior Cruciate Ligament-Bone Complex,” Med. Eng. Phys., 21, pp. 95–100.
Blevins,  F. T., Hecker,  A. T., Bigler,  G. T., Boland,  A. L., and Hayes,  W. C., 1994, “The Effects of Donor Age and Strain Rate on the Biomechanical Properties of Bone-Patellar Tendon-Bone Allografts,” Am. J. Sports Med., 22(3), pp. 328–33.
Haut,  R. C., 1985, “The Effect of a Lathyritic Diet on the Sensitivity of Tendon to Strain Rate,” J. Biomech. Eng., 107(2), pp. 166–74.
Haut,  R. C., 1983, “Age-Dependent Influence of Strain Rate on the Tensile Failure of Rat-Tail Tendon,” J. Biomech. Eng., 105(3), pp. 296–9.
Parry,  D. A., 1988, “The Molecular and Fibrillar Structure of Collagen and Its Relationship to the Mechanical Properties of Connective Tissue,” Biophys. Chem., 29(1-2), pp. 195–209.
Mikic,  B., Schalet,  B. J., Clark,  R. T., Gaschen,  V., and Hunziker,  E. B., 2001, “GDF-5 Deficiency in Mice Alters the Ultrastructure, Mechanical Properties and Composition of the Achilles Tendon,” J. Orthop. Res., 19(3), pp. 365–71.
Derwin,  K. A., and Soslowsky,  L. J., 1999, “A Quantitative Investigation of Structure-Function Relationships in a Tendon Fascicle Model,” J. Biomech. Eng., 121(6), pp. 598–604.
Parry,  D. A., and Craig,  A. S., 1977, “Quantitative Electron Microscope Observations of the Collagen Fibrils in Rat-Tail Tendon,” Biopolymers, 16(5), pp. 1015–31.
Trotter,  J. A., and Koob,  T. J., 1989, “Collagen and Proteoglycan in a Sea Urchin Ligament with Mutable Mechanical Properties,” Cell Tissue Res., 258, pp. 527–539.
Craig,  A. S., Birtles,  M. J., Conway,  J. F., and Parry,  D. A., 1989, “An estimate of the mean length of collagen fibrils in rat tail-tendon as a function of age,” Connect. Tissue Res., 19(1), pp. 51–62.
Blevins,  F. T., Djurasovic,  M., Flatow,  E. L., and Vogel,  K. G., 1997, “Biology of the Rotator Cuff Tendon,” Orthop. Clin. North Am. , 28(1), pp. 1–16.
Danielson,  K. G., Baribault,  H., Holmes,  D. F., Graham,  H., Kadler,  K. E., and Iozzo,  R. V., 1997, “Targeted Disruption of Decorin Leads to Abnormal Collagen Fibril Morphology and Skin Fragility,” J. Cell Biol., 136(3), pp. 729–43.
Scott,  J. E., 1988, “Proteoglycan-Fibrillar Collagen Interactions,” Biochem. J., 252(2), pp. 313–23.
Vogel,  K. G., and Heinegard,  D., 1985, “Characterization of Proteoglycans from Adult Bovine Tendon,” J. Biol. Chem., 260(16), pp. 9298–306.
Flint,  M. H., Craig,  A. S., Reilly,  H. C., Gillard,  G. C., and Parry,  D. A., 1984, “Collagen Fibril Diameters and Glycosaminoglycan Content of Skins--Indices of Tissue Maturity and Function,” Connect. Tissue Res., 13(1), pp. 69–81.
Pins,  G. D., Christiansen,  D. L., Patel,  R., and Silver,  F. H., 1997, “Self-Assembly of Collagen Fibers: Influence of Fibrillar Alignment and Decorin on Mechanical Properties,” Biophys. J., 73(4), pp. 2164–72.
Lam,  T. C., Frank,  C. B., and Shrive,  N. G., 1993, “Changes in the Cyclic and Static Relaxations of the Rabbit Medial Collateral Ligament Complex During Maturation,” J. Biomech., 26(1), pp. 9–17.
Chimich,  D., Shrive,  N. G., Frank,  C. B., Marchuk,  L., and Bray,  R., 1992, “Water Content Alters Viscoelastic Behavior of the Normal Adolescent Rabbit Medial Collateral Ligament,” J. Biomech., 25(8), pp. 831–7.
Liu,  X., Wu,  H., Byrne,  M., Jeffrey,  J., Krane,  S., and Jaenisch,  R., 1995, “A Targeted Mutation at the Known Collagenase Cleavage Site in Mouse Type I Collagen Impairs Tissue Remodeling,” Cell Biol., 130(1), pp. 227–237.
Bonadio,  J., Saunders,  T. L., Tsai,  E., Goldstein,  S. A., Morris-Wiman,  J., Brinkley,  L., Dolan,  D. F., Altschuler,  R. A., Hawkins,  J. E., and Bateman,  J. F., 1990, “Transgenic Mouse Model of the Mild Dominant Form of Osteogenesis Imperfecta,” Proc. Natl. Acad. Sci. U.S.A., 87(18), pp. 7145–9.
Derwin,  K. A., Soslowsky,  L. J., Kimura,  J. H., and Plaas,  A. H., 2001, “Proteoglycans and Glycosaminoglycan Fine Structure in the Mouse Tail Tendon Fascicle,” J. Orthop. Res., 19(2), pp. 269–77.
Clark,  R. T., Johnson,  T. L., Schalet,  B. J., Davis,  L., Gaschen,  V., Hunziker,  E. B., Oldberg,  A., and Mikic,  B., 2001, “GDF-5 Deficiency in Mice Leads to Disruption of Tail Tendon Form and Function,” Connect. Tissue Res., 42(3), pp. 175–186.
Christiansen,  D. L., Huang,  E. K., and Silver,  F. H., 2000, “Assembly of Type I Collagen: Fusion of Fibril Subunits and the Influence of Fibril Diameter on Mechanical Properties,” Matrix Biol., 19(5), pp. 409–420.
Mow, V. C., Flatow, E. L., and Ateshian, G. A., 2000, “Biomechanics,” in: Buckwalter, J. A., Einhorn, T. A., Simon, S. R., eds., Orthopaedic Basic Science, 2nd Ed., American Academy of Orthopaedic Surgeons, Chap. 5.
Lin,  T. W., White,  S. M., Robinson,  P. S., Derwin,  K. A., Plaas,  A. H., Iozzo,  R. V., and Soslowsky,  L. J., 2002, “Relating Extracellular Matrix Composition with Function -- A Study Using Transgenic Mouse Tail Tendon Fascicles,” Trans. Orthop. Res., 27, pp. 45.
Haut,  T. L., and Haut,  R. C., 1997, “The State of Tissue Hydration Determines the Strain-Rate Sensitive Stiffness of Human Patellar Tendon,” J. Biomech., 30(1), pp. 79–81.
Pioletti,  D. P., and Rakotomanana,  L. R., “On the Independence of Time and Strain Effects in the Stress Relaxation of Ligaments and Tendons,” J. Biomech., 33(12), pp. 1729–1732.
Woo,  S. L., Gomez,  M. A., and Akeson,  W. H., 1981, “The Time and History-Dependent Viscoelastic Properties of the Canine Medical Collateral Ligament,” J. Biomech. Eng., 103(4), pp. 293–8.

Figures

Grahic Jump Location
Fascicle prepared and mounted in test fixture. Bar=2 mm
Grahic Jump Location
Typical data and calculations for a slow (0.5%/s) test. Stress vs. optical strain data was fit with a nth order polynomial (4<n<9). Modulus was calculated as the slope of the polynomial at its inflection point (diamond).
Grahic Jump Location
Slow (0.5%/s) constant strain rate test mechanical properties. A: Maximum Load, B: Stiffness, C: Maximum Stress, D: Modulus. Bars are mean+standard deviation. CTL8=adult normal, C1TJ8=collagen accumulation, C1M8=collagen reduction, DKO8=decorin knockout, CTL3=immature normal. *=significantly different from CTL8 (p<0.003).
Grahic Jump Location
Fast (50%/s) constant strain rate test mechanical properties. A: Maximum Load, B: Stiffness, C: Maximum Stress, D: Modulus. Bars are mean+standard deviation. CTL8=adult normal, C1TJ8=collagen accumulation, C1M8=collagen reduction, DKO8=decorin knockout, CTL3=immature normal. *=significantly different from CTL8 (p<0.05).
Grahic Jump Location
Fast ramp/Slow ramp ratios of mechanical properties of each mouse group. A fast/slow ratio of 1.0 (thick horizontal bar) indicates no strain rate sensitivity. Bars are mean±standard deviation. CTL8=adult normal, C1TJ8=collagen accumulation, C1M8=collagen reduction, DKO8=decorin knockout, CTL3=immature normal. *=property strain rate sensitive (fast/slow ratio>1.0,p<0.05)#=significant difference from CTL8 (p<0.05).

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