Measurement and Simulation of Water and Methanol Transport in Algal Cells

[+] Author and Article Information
John R. Walsh, Kenneth R. Diller, Jerry J. Brand

Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712Currently at Organ Recovery Systems, Inc. Charleston, SC 29403, U.S.A.Department of Mechanical EngineeringMCDB-Biology, The University of Texas at Austin, Austin, TX 78712

J Biomech Eng 126(2), 167-179 (May 04, 2004) (13 pages) doi:10.1115/1.1688775 History: Received September 02, 2003; Revised September 29, 2003; Online May 04, 2004
Copyright © 2004 by ASME
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Grahic Jump Location
Micrograph (800×) of C. texanum.
Grahic Jump Location
Boyle-van’t Hoff plot for C. texanum cells exposed to hypertonic sucrose solutions at ambient temperature and pressure under equilibrium conditions. Cell volume is normalized to the isotonic volume, Viso, and expressed as a function of the inverse extracellular solute concentration normalized to the isotonic osmolality of 58 mOsm. The theoretical inactive volume of the cell was 38% as indicated by the intercept of the linear regression. n=16 independent measurements of individual cells. Data represent average ±SEM.
Grahic Jump Location
Equilibrium osmotic behavior of C. texanum across the entire range of sucrose solutions tested (0–1250 mOsm). Cell volume is influenced by the elastic cell wall and osmotic effects until incipient plasmolysis (250 mOsm) at which point cell volume is governed by Boyle-van’t Hoff behavior alone (dashed line-no symbol). Volume data is normalized to the full turgor volume Vfull.n=37.
Grahic Jump Location
Static volumetric response of C. texanum cells exposed to dilute sucrose solutions from 0–250 mOsm. The expressed cell volume ΔV is given by ΔV=Vfull−V, where Vfull is the volume at full turgor, and V is the equilibrium cell volume at each point (n=21 independent measurements of individual cells). Data represent average ±SEM.
Grahic Jump Location
Computed hydrostatic turgor pressure in C. texanum shown as a function of expressed cell volume ΔV (Vfull−V) normalized to the volume at full turgor, Vfull.n=21 independent measurements of individual cells
Grahic Jump Location
Arrhenius plots for hydraulic conductivity, Lp, and methanol permeability, ω, in C. texanum as computed for the three- and two-parameter models with a simultaneous inverse solution of the respective governing equations. The plots show the natural logarithm of Lp and ω, as a function of the reciprocal of absolute temperature. Open symbols represent average values generated by each model ±SEM. n=15.
Grahic Jump Location
Arrhenius plots for hydraulic conductivity, Lp, and methanol permeability, ω, in C. texanum for data generated with the three-parameter model using the zero-time method. Average permeability values are plotted as natural logarithms against the reciprocal of absolute temperature. Data indicated by filled diamonds were obtained at temperatures for which there were measurements of only Lp values to demonstrate potential conformational changes in membrane phospholipids as they undergo a phase transition occurring at just below the recorded growth temperature. Data represent average ±SEM. n=12(n=18 for all points data).
Grahic Jump Location
Comparison of osmotic model simulations and experimental data at (a) 25°C, (b) 15°C, and (c) −3°C for C. texanum cells exposed to 500 mOsm sucrose (t=0 s) and then (t=1,700 s for 25°C), (t=1900 s for 15°C), (t=3000 s for −3°C) to a solution containing 200 mOsm methanol and 300 mOsm sucrose. n=3.



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