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TECHNICAL PAPERS

Effects of Cyclic Stress on the Mechanical Properties of Cultured Collagen Fascicles from the Rabbit Patellar Tendon

[+] Author and Article Information
Ei Yamamoto

Biomechanics Laboratory, Department of Mechanical Engineering and Biomimetics, School of Biology-Oriented Science and Technology, Kinki University, Naga, Wakayama 649-6493, Japan

Susumu Tokura

Ishikawajima-Harima Heavy Industry, Yokohama, Kanagawa 235-8501, Japan

Kozaburo Hayashi

Biomechanics Laboratory, Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan

J Biomech Eng 125(6), 893-901 (Jan 09, 2004) (9 pages) doi:10.1115/1.1634286 History: Received February 15, 2002; Revised July 07, 2003; Online January 09, 2004
Copyright © 2003 by ASME
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References

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Figures

Grahic Jump Location
Apparatus for the culture of collagen fascicles under cyclic load conditions. Four fascicles can be cultured at the same time. They are immersed in Dulbecco’s modified Eagle medium (DMEM) of 37°C supplemented with 10% fetal calf serum (FCS), 100 U/ml penicillin, and 100 μg/ml streptomycin in a humidified chamber containing a gas mixture of 5% CO2 and 95% air. A linear actuator is used for the application of load, which is continuously monitored with a load cell.
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Definition of crimp angle, crimp length, and wave length 19. Crimp length was defined as the distance between the top and bottom points of a fibril. Crimp angle was defined as the angle between the line connecting the top and bottom points and the line connecting neighboring two middle points. Wave length was calculated from the crimp angle and crimp length.
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Typical stress-strain curves of the fascicles cultured under different applied stresses (σA) for one (a) and two weeks (b), and averaged curves of control and non-loaded fascicles. These stress-strain curves were almost linear between 2% and 5% strain, with toe regions under 2% strain.
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Applied peak stress versus tangent modulus of cultured collagen fascicles. There were statistically significant correlations (r=correlation coefficient) between them in one-week, two-week, and pooled data; each relation was described by a quadratic curve. The quadratic function for the pooled data had the maximum modulus of 211.0 MPa at the applied peak stress of 1.9 MPa. The moduli at applied peak stresses between 0.9 and 2.8 MPa were within a range of control values.
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Applied peak stress versus tensile strength of cultured collagen fascicles. There were statistically significant correlations between them in one-week, two-week, and pooled data; each relation was described by a quadratic curve. The quadratic function for the pooled data had the maximum strength of 19.4 MPa at the applied peak stress of 1.8 MPa. The strength at applied peak stresses between 1.1 and 2.6 MPa were within a range of control values.
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Applied peak stress versus strain at failure of cultured collagen fascicles. Strain at failure was negatively correlated with applied stress, and the relations were statistically significant in one week data and pooled data for one and two weeks.
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Comparisons of tangent modulus (a), tensile strength (b), and strain at failure (c) among the control, non-loaded, and loaded (0 to 1.0, 1.0 to 2.7, and 2.7 to 4.0 MPa groups) fascicles. For the assessment of the effects of time and load condition, a two-way ANOVA was performed, followed by Dunnett analysis for multiple comparisons between the control and cultured groups.
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Examples of the photomicrographs of crimp morphology in a control fascicle (a) and the fascicles cultured under no load (b), 0.40 MPa (c), 1.89 (d) and 3.80 (e) for two weeks. Bars indicate 50 μm.
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Comparisons of the effects of the magnitude of stress on tangent modulus (a) and tensile strength (b) between cyclic and static stress conditions. The relations were obtained from the pooled data for one and two weeks. The relations for static stress conditions were reproduced from Yamamoto et al. 19.

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