Kinetics Study of Endogenous Heat Shock Protein 70 Expression

[+] Author and Article Information
Sihong Wang, Kenneth R. Diller, Shanti J. Aggarwal

Department of Biomedical Engineering, ENS612, 1 University Station C0800, The University of Texas at Austin, Austin, TX 78712-0238 Phone: (512) 471-7167 Fax: (512) 471-0616

J Biomech Eng 125(6), 794-797 (Jan 09, 2004) (4 pages) doi:10.1115/1.1632522 History: Received July 30, 2003; Revised August 11, 2003; Online January 09, 2004
Copyright © 2003 by ASME
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Measured specimen temperature (T(t)) during heating in an incubator calibration at 42°C (315K). Empirical curve is fit to data, T(t)=0.8755*Ln(t)+307.5, and R2=0.9854
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Comparison of the actual incubator heating time (tactl) with the effective heating time (teff) for A=8.6*1055 (s−1) and ΔE=3.543*105 (J/mole) at 42°C
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HSP70 expression on the western blot membrane of (a) 12-h post-heating with various heating times and (b) 1.5 h heating with various post-heating times
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Endogenous HSP70 Expression after various 42°C heating and 37°C recovery times
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HSP70 Expression at 42°C for 1.6 hours effective heating followed by 37°C post-heating, each data point is presented by its average±SEM (standard error mean) indicated by error bar, n=7
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HSP70 expression of continuous heating at 42°C without recovery time, each data point is presented by its average±SEM,n=4
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HSP70 expression at 40∼44°C heat shock for two hours followed by 14 hours 37°C recovery, each column bar is presented by its average±SEM,n=4




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