Effect of Thermal Damage and Biaxial Loading on the Optical Properties of a Collagenous Tissue

[+] Author and Article Information
J.-H. Jun, J. L. Harris, J. D. Humphrey, S. Rastegar

Department of Biomedical Engineering, Texas A&M University, College Station, TX

J Biomech Eng 125(4), 540-548 (Aug 01, 2003) (9 pages) doi:10.1115/1.1591202 History: Received May 26, 2000; Revised March 13, 2003; Online August 01, 2003
Copyright © 2003 by ASME
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Grahic Jump Location
Schema of the overall system, consisting of four computer controlled motors that stretch the specimen in orthogonal directions, two load cells for measuring applied forces, a CCD combined with custom software for monitoring the deformation of the specimen in a central region, a laser-integrating sphere system for measuring optical properties, and a temperature measurement and control device
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Side-view (panel a, top ) and top-view (panel b, bottom) line drawings of the device (not to scale). Specific components are: CCD—video camera, GS—glass slide, LS—laser, M1-M5—optical mirrors, IS—integrating sphere, SC—specimen chamber, SP—specimen, S1-S4—stepper motors, P1-P3—possible laser beam paths and CCD viewing path, and IO—fluid inlet/outlet ports.
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Time-histories for the natural heating rate (no temperature control), constant heating rate (controlled here at 1°C/min), and isothermal heating (controlled here at 75°C) of the specimen chamber measured at the specimen location
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Schema of the overall experimental protocol. The epicardium is removed from the LV, prepared for testing, mechanically preconditioned for 10 cycles from 2–80 grams biaxially, unloaded to find the reference configuration and then subjected to mechanical and optical testing. The specimen is then unloaded and immersed in a 65°C solution for a specified heating time τ, which allows biaxial shrinkage ξ(τ). After heating, the specimen is returned to a room temperature normal saline for a one hour “recovery” period after which the equilibrium shrinkage ξe is measured. The specimen is then preconditioned for 10 cycles and unloaded to measure the new reference configuration. This process is repeated several times to get information at a series of damage states for a single specimen.
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Cross line between the measured and calculated transmittances via Monte Carlo simulation: (a) three-dimensional view, (b) projected view.
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Cross line between the measured and calculated reflectances via Monte Carlo simulation: (a) three-dimensional view, (b) projected view
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Effect of thermal denaturation on scattering coefficients of epicardium under biaxial loading at green wavelength (542 nm)
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Similar to Fig. 7 except at the red wavelength (633 nm)
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Absorption coefficients of native and denatured epicardium under biaxial loading at green wavelength (542 nm)
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Similar to Fig. 9 except at the red wavelength (633 nm)
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Percent change in the reduced scattering coefficient at (a) the green wavelength and (b) the red wavelength
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Comparison in the percent change in optical and mechanical properties at five different thermal damage states




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