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TECHNICAL PAPERS: Cell

Biomechanical and Optical Characteristics of a Corneal Stromal Equivalent

[+] Author and Article Information
Elizabeth J. Orwin

Department of Engineering, Harvey Mudd College, Claremont, CA

Melinda L. Borene

Department of Biomedical Engineering, University of Minnesota, 312 Church Street SE, Minneapolis, MN 55455

Allison Hubel

Department of Mechanical Engineering, University of Minnesota, 111 Church Street SE, Minneapolis, MN 55455

J Biomech Eng 125(4), 439-444 (Aug 01, 2003) (6 pages) doi:10.1115/1.1589773 History: Received June 21, 2002; Revised April 01, 2003; Online August 01, 2003
Copyright © 2003 by ASME
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References

Figures

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Compressive stress as a function of time for a stromal equivalent (collagen sponge matrix with cells) after 7 days in culture. The sample was loaded at a 15% strain rate.
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Modulus as a function of time in culture for a stromal equivalent (collagen sponge matrix). N≥7 for day 0, 7, 14, and 21. For all other days, n=2.
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Normalized projected area of stromal equivalents as a function of time and matrix composition (collagen sponge matrix). N≥6.
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(a) Fraction of light transmitted, FT, at 70 nm; and (b) fraction of light transmitted, FT, at 400 nm as a function of time and matrix composition for the stromal equivalent (collagen sponge matrix). N≥6.
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DNA content of the stromal equivalent (collagen sponge matrix) as a function of time. N=3.
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Fraction of stromal fibroblasts expressing α-smooth muscle actin as a function time in culture for a stromal equivalent (collagen sponge matrix). Three different initial seeding densities were studied: 1×104,5×104, and 1×105 cells/cm3.N≥4.

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