Particle Deposition in Arteries Ex Vivo: Effects of Pressure, Flow, and Waveform

[+] Author and Article Information
Naomi C. Chesler

Department of Mechanical Engineering, University of Vermont, Burlington, VT 05405-0156

Omyekachi C. Enyinna

Biomedical Engineering Program, University of Vermont, Burlington, VT 05405-0156

J Biomech Eng 125(3), 389-394 (Jun 10, 2003) (6 pages) doi:10.1115/1.1572905 History: Received July 23, 2001; Revised February 03, 2003; Online June 10, 2003
Copyright © 2003 by ASME
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Ku,  D., 1997, “Blood Flow in Arteries,” Annu. Rev. Fluid Mech., 29, pp. 399–434.
Delfino,  A., , 1998, “Wall stresses in the carotid bifurcation: effects of wall non-homogeneity and correlation with intimal thickness,” J Vasc Invest, 4(2), pp. 61–71.
Ku,  D. N., , 1985, “Pulsatile flow and atherosclerosis in the human carotid bifurcation. Positive correlation between plaque location and low oscillating shear stress,” Arteriosclerosis (Dallas), 5(3), pp. 293–302.
Stary,  H. C., , 1994, “A definition of initial, fatty streak, and intermediate lesions of atherosclerosis. A report from the Committee on Vascular Lesions of the Council on Arteriosclerosis, American Heart Association,” Circulation, 89(5), pp. 2462–2478.
Frangos,  S. G., Gahtan,  V., and Sumpio,  B., 1999, “Localization of atherosclerosis: role of hemodynamics,” Arch. Surg. (Chicago), 134(10), pp. 1142–1149.
Sill,  H. W., , 1995, “Shear stress increases hydraulic conductivity of cultured endothelial monolayers,” Am. J. Physiol., 268(2 Pt 2), pp. H535–43.
Jo,  H., , 1991, “Endothelial albumin permeability is shear dependent, time dependent, and reversible,” Am. J. Physiol., 260(6 Pt 2), pp. H1992–6.
Berceli,  S. A., , 1990, “Hemodynamics and low density lipoprotein metabolism. Rates of low density lipoprotein incorporation and degradation along medial and lateral walls of the rabbit aorto-iliac bifurcation,” Arteriosclerosis (Dallas), 10(5), pp. 686–694.
Zhao,  S. Z., , 2000, “Blood flow and vessel mechanics in a physiologically realistic model of a human carotid arterial bifurcation,” J. Biomech., 33(8), pp. 975–984.
Guretzki,  H. J., , 1994, “Atherogenic levels of low density lipoprotein alter the permeability and composition of the endothelial barrier,” Atherosclerosis, 107(1), pp. 15–24.
Cotran, R., Kutran, V., and Robbins, S., 1989, Robbins Pathologic Basis of Disease, 4th ed., W. B. Saunders Co., Philadelphia, PA.
Chesler,  N. C., , 1998, “Simplified ex vivo artery culture techniques for porcine arteries,” J Vasc Invest, 4, pp. 123–127.
Chesler,  N. C., Ku,  D. N., and Galis,  Z. S., 1999, “Transmural pressure induces matrix metalloproteinase and matrix degrading activity in porcine arteries ex vivo,” Am. J. Physiol., 277, (Heart Circ Physiol 46): pp. H2002–H2009.
Meyer,  G., Merval,  R., and Tedgui,  A., 1996, “Effects of pressure-induced stretch and convection on low-density lipoprotein and albumin uptake in the rabbit aortic wall,” Circ. Res., 79(3), pp. 532–540.
Lever,  M. J., and Jay,  M. T., 1993, “Convective and diffusive transport of plasma proteins across the walls of large blood vessels,” Front Med. Biol. Eng., 5(1), pp. 45–50.
Rome,  J. J., , 1994, “Anatomic barriers influence the distribution of in vivo gene transfer into the arterial wall,” Arterioscler. Thromb., 14, pp. 148–161.
MacLennan, M., et al., 2000, “Pressure increases particle uptaken in human saphenous vein,” Advances in Bioengineering, BED-Vol 48, ASME, New York, pp. 177–178.
Mann,  M. J., , 1999, “Pressure-mediated oligonucleotide transfection of rat and human cardiovascular tissues,” Proc. Natl. Acad. Sci. U.S.A., 96(11), pp. 6411–6416.
Lever,  M. J., Jay,  M. T., and Coleman,  P. J., 1996, “Plasma protein entry and retention in the vascular wall: possible factors in atherogenesis,” Can. J. Physiol. Pharmacol., 74(7), pp. 818–23.


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Ex vivo perfusion system for pulsatile waveform, variable pressure.
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Representative flowrate (A) and pressure (B) generated during steady and pulsatile perfusion of a porcine carotid artery ex vivo at a mean pressure of 100 mm Hg.
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Confocal laser microscopy images of vessels perfused with microspheres under steady (A, B) and pulsatile (C, D) conditions. Images A and C are control (no-flow), B and D are experimental (flow). Probes fluoresce in the red wavelength; cell nuclei fluoresce green. Images were taken at 10× magnification.
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Sphere area to intimal surface area ratio quantified from conofocal laser microscopy images of vessels perfused under steady (white), pulsatile (light gray) and oscillatory (dark gray) waveforms at mean pressures of 100 mmHg and 200 mmHg. Hatching differentiates flow (unhatched) from no flow (hatched). Bars represent mean±standard deviation.
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Baseline-adjusted sphere deposition data for steady, pulsatile and oscillatory waveforms to determine the additive effect of flow. Mean values for each group are shown.
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Scanning electron micrograph showing microspheres clustered and deposited along the intima.




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