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TECHNICAL PAPERS: Cell

Inertial Shear Forces and the Use of Centrifuges in Gravity Research. What is the Proper Control?

[+] Author and Article Information
Jack J. W. A. van Loon

Dutch Experiment Support Center (DESC), Oral Biology, ACTA Vrije Universiteit, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands

Erik H. T. E. Folgering

TNO Insitute of Applied Physics, Dept. of Mechanical Engineering, P.O. Box 155, 2600 AD Delft, The Netherlands

Carlijn V. C. Bouten

Eindhoven University of Technology, Dept. Biomedical Engineering, Biomechanics & Tissue Engineering, P.O. Box 513, 5600 MB Eindhoven, The Netherlands

J. Paul Veldhuijzen

ACTA Vrije Universiteit, Dept. Oral Biology, Group of Oral Cell Biology, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands

Theo H. Smit

Dept. Physics and Medical Technology, Vrije Universiteit Medical Center, Amsterdam, The Netherlands

J Biomech Eng 125(3), 342-346 (Jun 10, 2003) (5 pages) doi:10.1115/1.1574521 History: Received March 01, 2002; Revised January 01, 2003; Online June 10, 2003
Copyright © 2003 by ASME
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References

Mesland, D., Accensi, A., Alfermann, C, Bennett, J., Chin, D., Foeng, A., Franz, A., Gesta-Fernandez, J., Goldzahl, N., Helmke, H., Ives, J., Kruit, A., Soons, A., Burden, D., and Millican, S., 1987, “The Biorack Facility and its performance during the D1 spacelab mission,” ESA publication SP-1091, pp. 9–26.
Patrick, C.W., Sampath, R., and McIntire, L.V., 1995, “Fluid Shear Stress Effects on Cellular Function,” In: Biomedical Research Handbook: Tissue Engineering Section. eds., B. Palsson and J.A. Hubbell, CRC Press Inc., pp. 1626–1645.
Weinbaum,  S., Cowin,  S. C., and Zeng,  Y., 1994, “A Model for the Excitation of Osteocytes by Mechanical Loading-Induced Bone Fluid Shear Stresses,” J. Biomech., 27, pp. 339–60.
Klein, Nulend,  J., Van der Plas,  A., Semeins,  C. M., Ajubi,  N. E., Frongos,  J. A., Nijweide,  P. J., and Burger,  E. H., 1995, “Sensitivity of Osteocytes to Biomechanical Stress in vitro,” FASEB J., 9, pp. 441–45.
Knight,  M., Lee,  D., and Bader,  D., 1996, “Discription of Chondrocyte Deformation in Compressed Agarose Gel Using Confocal Microscopy,” Cellular Engineering, 1, pp. 97–102.
Zang,  Z., Ferenczi,  M., Lush,  A., and Thomas,  C., 1991, “A Novel Micromanipulation Technique for Measuring the Bursting Strength of a Single Mammalian Cell,” Applied Microbiology Biotechnology 36, pp. 208–210.
Grattarola, M., Ricci, D., Tedesco, M., 1996, “Atomic Force Microscopy on Cells Adhering to a Substrate: A Tool for Cellular Engineering,” Cellular Engineering IEEE, pp. 2053–54.
Yoshikawa,  Y., Yasuike,  T., Yagi,  A., and Yamada,  T., 1999, “Transverse Elasticity of Myofibrils of Rabbit Skeletal Muscle Studied by Atomic Force Microscopy,” Biochem. Biophys. Res. Commun., 256, pp. 13–19.
Driss-Ecole, D., Schoevaert, D., Noin, M., and Perbal, G., 1994, “Densitometric Analysis of Nuclear DNA Content in Lentil Roots Grown in Space,” Biology of the Cell 81, pp. 59–64.
Yu,  F., Driss-Ecole,  D., Rembur,  J., Legue,  V., and Perbal,  G., 1999, “Effects of Microgravity on the Cell Cycle in the Lentil Root,” Physiol. Plant., 105, pp. 171–178.
Schmitt,  D. A., Hatton,  J. P., Emond,  C., Chaput,  D., Paris,  H., Levade,  T., Cazenave,  J.-P., and Schaffar,  L., 1996, “The Distribution of Protein Kinase C in Human Leukocytes is Altered in Microgravity,” FASEB J., 10, pp. 1627–34.
Pross,  H. D., and Kiefer,  J., 1999, “Repair of Cellular Radiation Damage in Space Under Microgravity Conditions,” Radiat. Environ. Biophys., 38, pp. 133–138.
Pollard,  E. C., 1965, “Theoretical Studies on Living Systems in the Absence of Mechanical Stress,” J. Theor. Biol., 8, pp. 113–123.
Pollard, E. C., 1971, “Physical Determinants of Receptor Mechanisms,” In: Gravity and Organism. eds., A. Gordon, N. J. Cohen, Univ. Chicago Press, pp 25–34.
Albrecht-Buehler,  G., 1991, “Possible Mechanisms of Indirect Gravity Sensing by Cells,” American Society for Gravitational and Space Biology Bulletin, 4(2), pp. 25–34.
Todd,  P., 1989, “Gravity-Dependent Phenomena at the Scale of the Single Cell,” American Society for Gravitational and Space Biology Bulletin, 2, pp. 95–113.
Todd, P., 1990, “Physical Effects at the Cellular Level Under Altered Gravity conditions,” Cospar meeting, The Hague, S.10.1.6, pp. 1-6.
Todd, P., Klaus, D. M., Stodieck, L. S., Smith, J., Staehelin, L. A., Kacena, M., Manfredi, B., and Bukhari, A., 1996, “Cellular Responses to Gravity: Extracellular, Intracellular and In-between,” COSPAR Conference Reprint FI.5-0003, pp. 1–6.
Turing,  A. M., 1952, “The Chemical Basis of Morphogenesis,” Philos. Trans. R. Soc. London, 237, pp 37–72.
Kondepudi,  D. K., 1991, “Detection of Gravity through Nonequilibrium Mechanisms,” American Society for Gravitational and Space Biology Bulletin, 4(2), pp. 119–124.
Papaseit,  C., Pochon,  N., Tabony,  J., 2000, “Microtuble Self-Organization is Gravity Dependent,'’ Proceedings of the National Academy of Sciences, 97(15), pp. 8364-68.
Pierson,  D., and Moss,  F., 1995, “Detecting Periodic Unstable Points In Noisy Chaotic and Limit Cycle Attractors with Applications to Biology,” Phys. Rev. Lett., 75(11), pp. 2124–2127.
Greenwood,  P. E., Ward,  L. M., Russell,  D. F., Neiman,  A., and Moss,  F., 2000, “Stochastic Resonance Enhances the Electrosensory Information Available to Paddlefish for Prey Capture,” Phys. Rev. Lett., 84(20), pp. 4773–4776.
Van der Pol,  L., and Tramper,  J., 1998, “Shear Sensitivity of Animal Cells from a Culture Medium Perspective,” Trends Biotechnol., 16, pp. 323–328.
Barbee,  K. A., Mundel,  T., Lal,  R., and Davies,  P. F., 1995, “Subcellular Distribution of Shear Stress at the Surface of Flow-Aligned and Nonaligned Endothelial Monolayers,” American Journal of Physiology, 268 (Heart and Circulatory Physiology 37), pp. H1765-72.
Goodwin,  T. J., Prewett,  T. L., Wolf,  D. A., and Spauling,  G. F., 1993, “Reduced Shear Stress: A Major Component in the Ability of Mammalian Tissues to from Three-Dimensional Assemblies in Simulated Microgravity,” J. Cell. Biochem., 51, pp. 301–311.
Konings,  H., 1995, “Gravitropism of Roots: An Evaluation of Progress During the Last Three Decades,” Acta Botanica Neerlandica, 44(3), pp. 195–223.
Johnsson,  A., 1997, “Circumnutations: Results from Recent Experiments on Earth and in Space,” Planta, 203, pp. S147–158.
Hoson,  T., Kamisaka,  S., Masuda,  Y., Yamashita,  M., Buchen,  B., 1997, “Evaluation of the Three-Dimensional Clinostat as a Simulator of Weightlessness,” Planta, 203, pp. S187-197.
Kraft,  T. F. B., van Loon,  J. J. W. A., Kiss,  J. Z., 2000, “Plastid Position in Arabidopsis Columella Cells is Similar in Microgravity and on a Random-Positioning Machine,” Planta, 211(3), pp. 415–422

Figures

Grahic Jump Location
Geometry of an Experiment Container (EC) accommodated on a centrifuge and forces within such a rotating system on board a spacecraft in free fall. The centrifuge radius, A, is defined as the distance from the center of rotation to the center of the EC. The minimum radius, B, is the distance from the center of rotation to the center-inner wall of the EC. The maximum radius, C, is the distance from the center of rotation to the outer wall of the EC. Width, D, is the maximum lateral width of an EC. E is EC depth. The force of gravity, Fg, increases radially from the center of centrifugation. The inertial shear force, Fi, increases laterally from the center of centrifugation as depicted in the right EC along a plane surface with a schematic monolayer of cells. Fi is the total resulting force.
Grahic Jump Location
Graphical representation of gravity and inertial shear accelerations as they will be generated in a Type-I EC accommodated on the small centrifuge of the Biopack facility. A: Gravity accelerations. B: Inertial shear. C: Percentage gravity acceleration of total acceleration. The horizontal plate indicates an arbitrary level of 95% gravity acceleration. D: Percentage shear acceleration over total acceleration. All values below the arbitrary plain division indicate the surface area within an experiment container where less than 5% of the total acceleration generates inertial shear.
Grahic Jump Location
Shear strains calculated from a finite element model for an idealized homogeneous isotropic cell accelerated in the center plane (center) of a Type-I experiment container in the Biopack small centrifuge running at 1×g, versus a similar cell located at x = 20 mm from the center plane (border). The absolute deformation of the cell is small, but the peak shear strain in the eccentric cell is more than three times higher than in the cell in the central position (7.98 μstrain vs. 2.37 μstrain, respectively). The related peak shear stress in the eccentrically located cell (0.027 Pa) is likely large enough to provoke a biological response.
Grahic Jump Location
The distribution of non-adherent cells in a 1×g static on-ground centrifuge (A and C) or on a 1×g on-board centrifuge (B and D), in sample chambers of different surface geometry. Note that the mark for ’center of rotation’ and the curvature of the chamber are for clarity of the drawing not on the same scale.

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