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TECHNICAL PAPERS

A Modified Micropipette Aspiration Technique and Its Application to Tether Formation From Human Neutrophils

[+] Author and Article Information
Jin-Yu Shao, Jinbin Xu

Department of Biomedical Engineering, Washington University, Saint Louis, MO 63130

J Biomech Eng 124(4), 388-396 (Jul 30, 2002) (9 pages) doi:10.1115/1.1486469 History: Received September 01, 2001; Revised April 01, 2002; Online July 30, 2002
Copyright © 2002 by ASME
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References

Shao,  J.-Y., Ting-Beall,  H. P., and Hochmuth,  R. M., 1998, “Static and Dynamic Lengths of Neutrophil Microvilli,” Proc. Natl. Acad. Sci. U.S.A., 95, pp. 6797–6802.
Schmidtke,  D. W., and Diamond,  S. L., 2000, “Direct Observation of Membrane Tethers Formed During Neutrophil Attachment to Platelets or P-selectin Under Physiological Flow,” J. Cell Biol., 149, pp. 719–729.
Edwards, S. W., 1994, Biochemistry and Physiology of the Neutrophil, Cambridge University Press, New York.
Evans,  E., and Yeung,  A., 1994, “Hidden Dynamics in Rapid Changes of Bilayer Shape,” Chem. Phys. Carbon, 73, pp. 39–56.
Waugh,  R. E., and Bauserman,  R. G., 1995, “Physical Measurements of Bilayer-skeletal Separation Forces,” Ann. Biomed. Eng., 23, pp. 308–321.
Hochmuth,  R. M., Shao,  J.-Y., Dai,  J., and Sheetz,  M. P., 1996, “Deformation and Flow of Membrane into Tethers Extracted From Neuronal Growth Cones,” Biophys. J., 70, pp. 358–369.
Shao,  J.-Y., and Hochmuth,  R. M., 1996, “Micropipette Suction for Measuring Piconewton Forces of Adhesion and Tether Formation from Neutrophil Membranes,” Biophys. J., 71, pp. 2892–2901.
Levin,  J. D., Ting-Beall,  H. P., and Hochmuth,  R. M., 2001, “Correlating the Kinetics of Cytokine-induced E-selectin Adhesion and Expression on Endothelial Cells,” Biophys. J., 80, pp. 656–667.
Shao,  J.-Y., and Hochmuth,  R. M., 1999, “Mechanical Anchoring Strength of L-selectin, β2 Integrins and CD45 to Neutrophil Cytoskeleton and Membrane,” Biophys. J., 77, pp. 587–596.
Engstrom,  K. G., Moller,  B., and Meiselman,  H. J., 1992, “Optical Evaluation of Red Blood Cell Geometry Using Micropipette Aspiration,” Blood Cells, 18, pp. 241–258.
Evans,  E., and Yeung,  A., 1989, “Apparent Viscosity and Cortical Tension of Blood Granulocytes Determined by Micropipette Aspiration,” Biophys. J., 56, pp. 151–160.
Ting-Beall,  H. P., Lee,  A. S., and Hochmuth,  R. M., 1995, “Effect of Cytochalasin D on the Mechanical Properties and Surface Morphology of Passive Human Neutrophils,” Ann. Biomed. Eng., 23, pp. 666–671.
Tsai,  M. A., Frank,  R. S., and Waugh,  R. E., 1994, “Passive Mechanical Behavior of Human Neutrophils: Effect of Cytochalasin B,” Biophys. J., 66, pp. 2166–2172.
Waugh, R. E., and Hochmuth, R. M., 1995, “Mechanics and Deformability of Hematocytes,” The Biomedical Engineering Handbook, Bronzino, J. D., ed. CRC Press, Inc., Boca Raton, FL, pp. 474–486.
Gelles,  J., Schnapp,  B. J., and Sheetz,  M. P., 1988, “Tracking Kinesin-driven Movements with Nanometer-scale Precision,” Nature (London), 331, pp. 450–453.
Devore, J. L., 1995, Probability and Statistics for Engineering and the Sciences, 4th Ed., Wadsworth, Inc., Belmont, CA.
Zar, J. H., 1999, Biostatistical Analysis, 4th Ed., Prentice Hall, Upper Saddle River, N.J.
Chesla,  S. E., Selvaraj,  P., and Zhu,  C., 1998, “Measuring Two-dimensional Receptor-ligand Binding Kinetics by Micropipette,” Biophys. J., 75, pp. 1553–1572.
Piper,  J. W., Swerlick,  R. A., and Zhu,  C., 1998, “Determining Force Dependence of Two-dimensional Receptor-ligand Binding Affinity by Centrifugation,” Biophys. J., 74, pp. 492–513.
Kaplanski,  G., Teysseire,  N., Farnarier,  C., Kaplanski,  S., Lissitzky,  J. C., Durand,  J. M., Soubeyrand,  J., Dinarello,  C. A., and Bongrand,  P., 1995, “IL-6 and IL-8 Production from Cultured Human Endothelial Cells Stimulated by Infection With Rickettsia Conorii Via a Cell-associated IL-1 Alpha-dependent Pathway,” J. Clin. Invest., 96, pp. 2839–2844.
Huber,  A. R., Kunkel,  S. L., Todd,  R. F., and Weiss,  S. J., 1991, “Regulation of Transendothelial Neutrophil Migration by Endogenous Interleukin-8,” Science, 254, pp. 99–102.
Lavkan,  A. H., Astiz,  M. E., and Rackow,  E. C., 1998, “Effects of Proinflammatory Cytokines and Bacterial Toxins on Neutrophil Rheologic Properties,” Crit. Care Med., 26, pp. 1677–1682.
Kucik,  D. F., Dustin,  M. L., Miller,  J. M., and Brown,  E. J., 1996, “Adhesion-activating Phorbol Ester Increases the Mobility of Leukocyte Integrin LFA-1 in Cultured Lymphocytes,” J. Clin. Invest., 97, pp. 2139–2144.
Liu,  Z.-Y., Young,  J.-I., and Elson,  E. L., 1987, “Rat Basophilic Leukemia Cells Stiffen When They Secrete,” J. Cell Biol., 105, pp. 2933–2943.
Diamond,  M. S., and Springer,  T. A., 1993, “A Subpopulation of mac-1(CD11b/CD18) Molecules Mediates Neutrophil Adhesion to ICAM-1 and Fibrinogen,” J. Cell Biol., 120, pp. 545–556.
Springer,  T. A., 1995, “Traffic Signals on Endothelium for Lymphocyte Recirculation and Leukocyte Emigration,” Annu. Rev. Physiol., 57, pp. 827–872.
Tsai,  M. A., Frank,  R. S., and Waugh,  R. E., 1993, “Passive Mechanical Behavior of Human Neutrophils: Power-law Fluid,” Biophys. J., 65, pp. 2078–2088.
Drury,  J. L., and Dembo,  M., 1999, “Hydrodynamics of Micropipette Aspiration,” Biophys. J., 76, pp. 110–128.
Kuo,  S. C., and Sheetz,  M., 1992, “Optical Tweezers in Cell Biology,” Trends Cell Biol., 2, pp. 116–118.
Williams,  T. E., Nagarajan,  S., Selvaraj,  P., and Zhu,  C., 2000, “Concurrent and Independent Binding of Fcgamma Receptors IIa and IIIb to Surface-Bound IgG,” Biophys. J., 79, pp. 1867–1875.
Williams,  T. E., Selvaraj,  P., and Zhu,  C., 2000, “Concurrent Binding to Multiple Ligands: Kinetic Rates of CD16b for Membrane-Bound IgG1 and IgG2,” Biophys. J., 79, pp. 1858–1866.

Figures

Grahic Jump Location
A diagram of the modified micropipette manipulation system. Another micropipette can be set up on the other side of the chamber if two micropipettes are needed.
Grahic Jump Location
A video micrograph showing tether formation with two micropipettes. The cell shown here is a human neutrophil stimulated with 50 ng/ml PMA.
Grahic Jump Location
Two video micrographs showing how to measure the residual cortical tension of a neutrophil treated with 100 μM cytochalasin D by aspirating the non-blebby region of the same cell with two micropipettes whose diameters are 5.2 μm and 3.3 μm respectively
Grahic Jump Location
The displacement of a latex bead fixed on a glass cover slip over time. The displacement was tracked with BeadPro8 and BeadPro18 respectively at a speed of 30 frames/second. Shown by the legends are the average bead positions and the standard deviations.
Grahic Jump Location
The displacement of a latex bead moving towards and away from the opening of a micropipette under an alternating net pressure of 1 pN/μm2 . Here, the velocities of the bead are ∼4 μm/s.
Grahic Jump Location
Tether formation from passive neutrophils with the neutrophil as the transducer (circle) and the bead as the transducer (cross). The solid line represents a linear fit through the circles and the dashed line represents a linear fit through the crosses. Each cross represents an average of two to eight tethers from the same cell at a certain pressure. The error bars stand for the standard deviations.
Grahic Jump Location
Tether formation from neutrophils stimulated with 25 ng/ml IL-8. The solid line represents Eq. (5) and each cross represents an average of three to seven tethers from the same cell at a certain pressure. The error bars stand for the standard deviations.
Grahic Jump Location
Tether formation from neutrophils stimulated with 50 ng/ml PMA. The solid line represents Eq. (5) and each cross represents an average of five to ten tethers from the same cell at a certain pressure. The error bars stand for the standard deviations.
Grahic Jump Location
Tether formation from neutrophils treated with 100 μM cytochalasin D. The solid line represents Eq. (5) and each cross represents an average of one to seventeen tethers from the same cell at a certain pressure. The error bars stand for the standard deviations.
Grahic Jump Location
A doublet of two spherical beads on a flat surface. OA and OB are the centers of the two beads. A and B are the projections of OA and OB on the flat surface respectively. P is the projection of OA on OBB.

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