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TECHNICAL BRIEFS

A New Flow Chamber for the Study of Shear Stress and Transmural Pressure Upon Cells Adhering to a Porous Biomaterial

[+] Author and Article Information
Roxana Chotard-Ghodsnia, Agnès Drochon, Reinhard Grebe

UMR CNRS 6600 Biomécanique et Génie Biomédical, Université de Technologie de Compiègne, BP 20529, 60205 Compiègne cedex, France

J Biomech Eng 124(2), 258-261 (Mar 29, 2002) (4 pages) doi:10.1115/1.1448520 History: Received July 01, 2000; Revised May 01, 2001; Online March 29, 2002
Copyright © 2002 by ASME
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References

Levesque,  M. J., and Nerem,  R. M., 1985, “The Elongation and Orientation of Cultured Endothelial Cells in Response to Shear Stress,” ASME J. Biomech. Eng., 107, pp. 341–347.
Dewey,  C. F., Bussolari,  S. R., Gimbrone,  M. A., and Davies,  P. F., 1981, “The Dynamic Response of Vascular Endothelial Cells to Fluid Shear Stress,” ASME J. Biomech. Eng., 103, pp. 177–185.
Truskey,  G. A., and Pirone,  J. S., 1990, “The Effect of Fluid Shear Stress Upon Cell Adhesion to Fibronectin-Treated Surfaces,” J. Biomed. Mater. Res., 24, pp. 1333–1353.
Truskey,  G. A., and Proulx,  T. L., 1993, “Relationship Between 3T3 Cell Spreading and the Strength of Adhesion on Glass and Silane Surfaces,” Biomaterials, 14, 4, pp. 243–254.
Acevedo,  A. R., Bowser,  S. S., Gerritsen,  M. E., and Bizios,  R., 1993, “Morphological and Proliferative Responses of Endothelial Cells to Hydrostatic Pressure: Role of Fibroblast Growth Factor,” J. Cell Physiol. , 157, pp. 603–614.
Thoumine,  O., Nerem,  R. M., and Girard,  P. R., 1995, “Oscillatory Shear Stress and Hydrostatic Pressure Modulate Cell-Matrix Attachment Proteins in Cultured Endothelial Cells,” In Vitro Cell Dev. Biol., 31A, pp. 45–54.
Tarbell,  J. M., Demaio,  L., and Zaw,  M. M., 1999, “Effect of Pressure on Hydraulic Conductivity of Endothelial Monolayers: Role of Endothelial Cleft Shear Stress,” J. Appl. Physiol., 87(1), pp. 261–268.
Jaffrin,  M. J., Reach,  G., and Notelet,  D., 1988, “Analysis of Ultrafiltration and Mass Transfer in Bioartificial Pancreas,” ASME J. Biomech. Eng., 110, pp. 1–10.
Höniger,  J., Darquy,  S., Reach,  G., Muscat,  E., Thomas,  M., and Collier,  C., 1994, “Preliminary Report on Cell Encapsulation in a Hydrogel Made of a Biocompatible Material, AN69, for the Development of a Bioartificial Pancreas,” Int. J. Artif. Organs, 17, pp. 46–52.
Artmann,  G. M., 1995, “Microscopic Photometric Quantification of Stiffness and Relaxation Time of Red Blood Cells in a Flow Chamber,” Biorheology, 32, 5, pp. 553–570.
Ofsthun,  N. J., and Leypoldt,  J. K., 1995, “Ultrafiltration and Backfiltration During Hemodialysis,” Int. J. Artif. Organs, 19, 11, pp. 1143–1161.
Lew,  H. S., and Fung,  Y. C., 1969, “Flow in an Occluded Circular Cylindrical Tube With Permeable Wall,” J. Appl. Mathematics and Physics, 20, pp. 750–766.
Deen, W. M., 1998, Analysis of Transport Phenomena, Oxford University Press, New York, pp. 274–275.
Mulvihill,  J., Cazenave,  J-P., Mazzucotelli,  J-P., Crost,  T., Collier,  C., Renaux,  J-L., and Pusineri,  C., 1992, “Minimodule Dialyser for Quantitative Ex Vivo Evaluation of Membrane Haemocompatibility in Humans: Comparison of Acrylonitrile Copolymer, Cuprophan and Polysulfone Hollow Fibers,” Biomaterials, 13, 8, pp. 527–535.
Lundberg,  L., Stegmayr,  B. G., and Wehle,  B., 1994, “Backdiffusion or Bicarbonate May Stimulate Complement Activation During Haemodialysis With Low-Flux Membranes,” Int. J. Artif. Organs, 17, 3, pp. 131–136.
Sill, H. W., Chang, Y. S., Artman, J. R., Frangos, J. A., Hollis, T. H., and Tarbell, J. M., 1995, “Shear Stress Increases Hydraulic Conductivity of Cultured Endothelial Monolayers,” Am. J. Physiol., 268 , (Heart Circ.Physiol. 37), H535–H543.

Figures

Grahic Jump Location
Top view of the opened flow chamber. The nylon screen (gray colored) is positioned on the bottom part and is surrounded by the bottom silicone ring. The porous biomaterial is then positioned on this nylon screen. To assemble, the upper part is turned over and fitted on the bottom part with screws.
Grahic Jump Location
Tangential mode flow system
Grahic Jump Location
(a) Filtrate flow rate, Qf, as a function of mean transmural pressure, TMPm, (b) Tangential flow rate Qout, as a function of the pressure drop in the chamber, ΔP

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