Tensile Mechanical Properties of Three-Dimensional Type I Collagen Extracellular Matrices With Varied Microstructure

Blayne A. Roeder; Klod Kokini; Jennifer E. Sturgis; J. Paul Robinson; Sherry L. Voytik-Harbin

doi:10.1115/1.1449904

Journal of Biomechanical Engineering | Volume 124 | Issue 2 | TECHNICAL PAPERS

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TECHNICAL PAPERS

Tensile Mechanical Properties of Three-Dimensional Type I Collagen Extracellular Matrices With Varied Microstructure

Blayne A. Roeder, Student Mem. ASME, Klod Kokini, Mem. ASME, Jennifer E. Sturgis, J. Paul Robinson and Sherry L. Voytik-Harbin

[+] Author Affiliations

Blayne A. Roeder

School of Mechanical Engineering, Purdue University, West Lafayette, IN 47907-1288, e-mail: blayne@ecn.purdue.edu

Klod Kokini

School of Mechanical Engineering/Department of Biomedical Engineering, Purdue University, West Lafayette, IN 47907-1288 , e-mail: kokini@ecn.purdue.edu

Jennifer E. Sturgis

Department of Basic Medical Sciences, Purdue University, West Lafayette, IN 47907-1515, e-mail: jennie@flowcyt.cyto.purdue.edu

J. Paul Robinson, Sherry L. Voytik-Harbin

Department of Basic Medical Sciences/Department of Biomedical Engineering, Purdue University, West Lafayette, IN 47907-1515

J Biomech Eng 124(2), 214-222 (Mar 29, 2002) (9 pages) doi:10.1115/1.1449904 History: Received May 31, 2001; Revised October 08, 2001; Online March 29, 2002

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Construction of the mold used to prepare collagen matrix test specimens (a). Schematic showing the dimensions of the collagen matrix test specimen with polypropylene mesh reinforcement (b).

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Cut away view of the experimental setup used for mechanical testing of collagen matrices. Collagen matrices were tested while submerged in PBS, pH 7.4 at 37°C.

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Representative stress-strain curve of a collagen matrix (2 mg/mL, pH 7.4) tested at a strain rate of 38.5 percent/min. The stress strain curve can be separated into three distinct regions designated “toe,” “linear,” and “failure.” Determinations of linear modulus, failure stress and failure strain are demonstrated.

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Representative 3D reconstructed confocal reflection image of a matrix prepared from purified type I collagen (1 mg/mL, pH 7.4)

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Confocal reflection images comparing the microstructure of collagen matrices prepared at concentrations of 0.3 mg/mL (a), 1 mg/mL (b), 2 mg/mL (c), and 3 mg/mL (d). An increase in fibril density was observed with increasing collagen concentration (10 μm bar is applicable to all images).

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Confocal reflection images comparing the microstructure of collagen matrices (2 mg/mL) prepared at pH of 6.0 (a), 7.0 (b), 8.0 (c), and 9.0 (d). Both fibril diameter and length were affected by pH. Fibrils formed at lower pH were shorter and thicker than those produced at higher pH (10 μm bar is applicable to all images).

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Time-lapse images demonstrating the tensile testing of a collagen matrix (2 mg/mL, pH 7.4) at a strain rate of 38.5 percent/min

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Effect of polymerization time on the linear modulus, failure stress and failure strain of 1.0 mg/mL (▴), 2.0 mg/mL (•), and 3.0 mg/mL (▪) collagen matrices (pH 7.4) tested at a strain rate of 38.5 percent/min. Exponential curve fits are shown for the linear modulus and failure stress data.

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Effect of polymerization time on the linear modulus, failure stress and failure strain of pH 6.0 (▾), pH 7.4 (•), and pH 9.0 (♦) collagen matrices (2.0 mg/mL) tested at a strain rate of 38.5 percent/min. Exponential curve fits are shown for the linear modulus and failure stress data.

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Effect of collagen concentration (0.3–3.0 mg/mL) on the linear modulus (•), failure stress (♦), and failure strain (○) of collagen matrices (pH 7.4) tested at 38.5 percent/min

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Effect of phosphate buffer pH (6.0–9.0) on the linear modulus (•), failure stress (♦), and failure strain (○) of collagen matrices (2 mg/mL) tested at a strain rate of 38.5 percent/min

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Effect of strain rate (19.2–385 percent/min) on the linear modulus (•), failure stress (♦), and failure strain (○) of collagen matrices (2 mg/mL, pH 7.4)

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Comparison of “true” stress (approximated) and “engineering” stress (calculated) as a function of strain for a collagen matrix (2mg/mL, pH 7.4)

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Tensile Mechanical Properties of Three-Dimensional Type I Collagen Extracellular Matrices With Varied Microstructure

References

Figures

Construction of the mold used to prepare collagen matrix test specimens (a). Schematic showing the dimensions of the collagen matrix test specimen with polypropylene mesh reinforcement (b).

Cut away view of the experimental setup used for mechanical testing of collagen matrices. Collagen matrices were tested while submerged in PBS, pH 7.4 at 37°C.

Representative 3D reconstructed confocal reflection image of a matrix prepared from purified type I collagen (1 mg/mL, pH 7.4)

Confocal reflection images comparing the microstructure of collagen matrices prepared at concentrations of 0.3 mg/mL (a), 1 mg/mL (b), 2 mg/mL (c), and 3 mg/mL (d). An increase in fibril density was observed with increasing collagen concentration (10 μm bar is applicable to all images).

Time-lapse images demonstrating the tensile testing of a collagen matrix (2 mg/mL, pH 7.4) at a strain rate of 38.5 percent/min

Effect of polymerization time on the linear modulus, failure stress and failure strain of 1.0 mg/mL (▴), 2.0 mg/mL (•), and 3.0 mg/mL (▪) collagen matrices (pH 7.4) tested at a strain rate of 38.5 percent/min. Exponential curve fits are shown for the linear modulus and failure stress data.

Effect of polymerization time on the linear modulus, failure stress and failure strain of pH 6.0 (▾), pH 7.4 (•), and pH 9.0 (♦) collagen matrices (2.0 mg/mL) tested at a strain rate of 38.5 percent/min. Exponential curve fits are shown for the linear modulus and failure stress data.

Effect of collagen concentration (0.3–3.0 mg/mL) on the linear modulus (•), failure stress (♦), and failure strain (○) of collagen matrices (pH 7.4) tested at 38.5 percent/min

Effect of phosphate buffer pH (6.0–9.0) on the linear modulus (•), failure stress (♦), and failure strain (○) of collagen matrices (2 mg/mL) tested at a strain rate of 38.5 percent/min

Effect of strain rate (19.2–385 percent/min) on the linear modulus (•), failure stress (♦), and failure strain (○) of collagen matrices (2 mg/mL, pH 7.4)

Comparison of “true” stress (approximated) and “engineering” stress (calculated) as a function of strain for a collagen matrix (2mg/mL, pH 7.4)

Tables

Errata

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