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TECHNICAL PAPERS

A Novel In-Vitro System for the Simultaneous Exposure of Bladder Smooth Muscle Cells to Mechanical Strain and Sustained Hydrostatic Pressure

[+] Author and Article Information
Karen M. Haberstroh, Natacha DePaola, Sarah A. Frommer, Rena Bizios

Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180

Martin Kaefer

Pediatric Urology, Riley Hospital for Children, Indianapolis, IN 46202

J Biomech Eng 124(2), 208-213 (Mar 29, 2002) (6 pages) doi:10.1115/1.1449903 History: Received August 03, 2000; Revised December 05, 2001; Online March 29, 2002
Copyright © 2002 by ASME
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References

Figures

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Schematic diagram of the hydrostrain system. A schematic diagram (not to scale) of the Hydrostrain System used to expose bladder smooth muscle cells simultaneously to mechanical strains (up to 25 percent) and hydrostatic pressure (40 cm H2O)
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Ovine bladder smooth muscle cells adherent onto the fibronectin-coated, flexible silastic membranes. Representative light micrographs illustrating bladder smooth muscle cells adherent onto either tissue culture polystyrene (Frame A) or onto the flexible, fibronectin-coated, silastic membranes (Frame B) under control conditions (that is, cells maintained under 0.3 cm H2O pressure but no mechanical strain under standard cell culture conditions) for 7 hours. These results provided evidence that the bladder smooth muscle cells used in the present study did adhere to the fibronectin-coated membranes; moreover, these cells expressed similar cell morphology on the flexible, silastic membranes and on the rigid, tissue-culture polystyrene. Stain: Coomassie Blue. Magnification: 25X.
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Normalized HB-EGF mRNA expression by ovine bladder smooth muscle cells simultaneously exposed to mechanical strain of up to 25 percent and to hydrostatic pressure of 40 cm H2O. HB-EGF mRNA levels of ovine bladder smooth muscle cells (in serum-free DMEM) on flexible, fibronectin-coated, silastic membranes simultaneously exposed to mechanical strain of up to 25 percent and to hydrostatic pressure of 40 cm H2O (bar C) under standard cell culture conditions increased (by more than 25 percent) compared to control cells maintained on flexible, silastic substrates under 0.3 cm H2O pressure but no mechanical strain (bar B). Data are mean values ±SEM of three experiments and were normalized with reference (that is, cells maintained on rigid, tissue-culture polystyrene substrates under 0.3 cm H2O pressure but no mechanical strain; bar A) values taken as 100 percent. * p<0.01 compared to control cells maintained on flexible, silastic substrates under 0.3 cm H2O pressure but no mechanical strain (bar B).
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Normalized collagen type I mRNA expression by ovine bladder smooth muscle cells simultaneously exposed to mechanical strain of up to 25 percent and to hydrostatic pressure of 40 cm H2O. Collagen Type I mRNA levels of ovine bladder smooth muscle cells (in serum-free DMEM) on flexible, fibronectin-coated, silastic membranes simultaneously exposed to mechanical strain of up to 25 percent and to hydrostatic pressure of 40 cm H2O (bar C) under standard cell culture conditions were similar to levels obtained from control cells maintained on flexible, silastic substrates under 0.3 cm H2O pressure but no mechanical strain (bar B). Data are mean values ±SEM of three experiments and were normalized with reference (that is, cells maintained on rigid, tissue-culture polystyrene substrates under 0.3 cm H2O pressure but no mechanical strain; bar A) values taken as 100 percent.
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Normalized collagen Type III mRNA expression by ovine bladder smooth muscle cells simultaneously exposed to mechanical strain of up to 25 percent and to hydrostatic pressure of 40 cm H2O. Collagen Type III mRNA levels of ovine bladder smooth muscle cells (in serum-free DMEM) on flexible, fibronectin-coated, silastic membranes simultaneously exposed to mechanical strains of up to 25 percent and to hydrostatic pressure of 40 cm H2O (bar C) under standard cell culture conditions increased by 13 percent compared to levels obtained from control cells maintained on flexible, silastic substrates under 0.3 cm H2O pressure but no mechanical strain (bar B). Data are mean values ±SEM of three experiments and were normalized with reference (that is, cells maintained on rigid, tissue-culture polystyrene substrates under 0.3 cm H2O pressure but no mechanical strain; bar A) values taken as 100 percent. * p<0.1 compared to control cells maintained on flexible, silastic substrates under 0.3 cm H2O pressure but no mechanical strain (bar B).

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